Exploration within a mature basin poses many challenges, not least how to best utilise resources and time to maximise success and reduce cost. Play-based exploration (PBE) provides a team-based approach to combine key aspects of the petroleum system into an integrated and wholistic view of basin prospectivity. While the PBE methodology is well established, it is not often applied to its full extent on a basin scale. After a period of declining exploration success in parts of the South Australia Cooper-Eromanga Basin, this study was undertaken by a dedicated regional geoscience team with the aim of rebuilding an understanding of the basin, based on first principles and stripping away exploration paradigms. The study area comprises an acreage position in the South Australian and Queensland Cooper-Eromanga Basins covering 70 000 km2 in which Senex Energy has 14 oil fields, has drilled more than 80 exploration wells and has acquired 2D and 3D seismic material. A plethora of proven and emerging plays exist within the acreage ranging from high productivity light sweet oil (Birkhead and Namur Reservoirs) to tight oil (Murta Formation), conventional gas (Toolachee/Epsilon and Patchawarra Formation), tight gas (Patchawarra Formation) and the emerging deep coal play (Toolachee and Patchawarra Coals). Play-based exploration methodologies incorporating the integration of seismic data, log and palynological data, structural analysis, geochemistry, 3D basin modelling, consistent well failure analysis and gross depositional environment maps have allowed the systematic creation of common risk segment maps at all play levels. This information is now actively utilised for permit management, business development, work program creation and portfolio management. This paper will present an example of the work focussing on the southern section of the South Australian Cooper-Eromanga Basin.