New conjugates of 18E-and 18D-glycyrrhizic acids (GAs) each containing two di-or D-methyl esters of L-aspartic acid in the carbohydrate part of the glycosides were synthesized by the activated ester method using the N-hydroxysuccinimide (HOSu) and N,Nc-dicyclohexylcarbodiimide. It was found that the conjugate of 18E-GA with Asp(OMe)(OMe) (4) at a concentration of 250 Pg/mL inhibited effectively RT of HIV-1 and the accumulation of virus antigen p24 in MT-4 cell culture (95-97%) and protected cells from the cytopathogenic action of the virus.The search for novel immunostimulators and antiviral agents with a new mechanism of action among natural compounds obtained from renewable plant resources and their modified forms is a burgeoning area of modern bioorganic and medicinal chemistry. This is explained by the broad distribution of HIV-infections, viral hepatitises B and C, and the emergence of new viral respiratory infections.