Tinospora is a genus within the family Menispermaceae renowned for its medicinal properties. The present study was undertaken to develop an effective protocol for optimum callus induction and organogenesis in Tinospora formanii, a rare endemic plant reported from Western Ghats of Kerala. Data of number of days required for callus induction, callus induction percentage, callus morphology, callus fresh weight and number of days required for shoot regeneration were recorded. Nodal segments from one year old plants were used as explants for callus induction and shoot regeneration. Number of days taken for callus induction in this medium was 5±0.28. Organogenic creamy brown friable callus developed. The fresh weight of the callus was 4.75±0.08 gm after 60 days of culture. Small green shoot buds developed from this callus after 65 days of culture which developed into shoots of about 2.5-3.0 cm length.