Plants are good source for the bioactive compounds and are used as traditional medicines. Phytochemical investigation of plants emphasizes in traditional medicines has yielded various bioactive compounds with different pharmacological activities. In the Hyacinthaceae family, Ledebouria genus is the weakly evergreen bulbs. Ledebouria hyderabadensis is a new toxin exist in the Hyderabad city of Telangana state, India. In the present study, we have carried out isolation of homoisoflavone from the underground bulbs of L. hyderabadensis and phytochemical screening of crude extracts of bulbs of L. hyderabadensis. The methanol extract of underground bulbs and isolated compound were screened for antimicrobial activity. Both the methanol extract and isolated compound Scillascillin shows significant antimicrobial activity.

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