This chapter focuses on alliances and resistances within niches at the regional scale, and on the specific processes which allow them to overcome barriers and enable an emerging transition. The case studies presented in this chapter are alternative agri-food networks (AAFNs) in three regions of Europe: short supply chains in Rennes Metropole (France); farmers markets in Pilsen (Czech Republic); and integration of winemaking and conventional tourism in Santorini (Greece). The assessment of the AAFNs demonstrates the varying sources of innovation, and forms of resistance adopted by regime actors to niche innovations.