The article presents experimental data on the influence of the feeding diet on the biochemical blood parameters of laying hens. As feed additives in the poultry diet were used: feed vitamin-mineral concentrate (FVMC) in the amount of 0.3% of the basic diet (BD) (1st experimental group), probiotic additive “Bacell-M” in the amount of 0.2% of BD (2nd experimental group), complex feed concentrate (CFC), containing FVMC and probiotic additive “Bacell-M”, in the amount of 0.3% of BD (3rd experimental group). The control group received only BD. It was found that the introduction of the feed additives into the diet of laying hens stimulated the metabolic processes in the poultry body, which was manifested by an increase in a number of biochemical constants. So, the level of total protein in the 1st experimental group increased by 34%, in the 2nd and 3rd experimental groups it increased in 1.43 and 1.42 times in comparison with the control group. The urea concentration exceeded the analogical values of the control poultry by 27% (1st experimental group), by 23.2% (2nd experimental group) and by 24.3% (3rd experimental group), respectively. The use of feed additives influenced the state of carbohydrate and lipid metabolism of hens, which was manifested by an increase in glucose concentration in the groups by 6.3%, 13.1% and 21.5% and by an increase of triglycerides by 13.2%, in 1.7 and in 2.0 times respectively. The inclusion of additives in the feeding schemes contributed to the correction of mineral metabolism, providing more complete absorption of calcium by the poultry, the increase of which in the experimental groups was by 16.2%, 1.68% and in 2.21 times, and also prevented the development of hepatocyte cytolysis, as indicated by a decrease in the activity of hepatoindicatory of enzymes: ALT decreased by 9.8%, 29.5% and in 1.85 times, AST decreased by 7.9%, 12.7% and 18.9%, respectively. The maximum positive effect in the indicators of the biochemical status of laying hens was observed when CFC was used in the diet, which was due to the synergy of FVMC and the Bacell-M probiotic additive.

The influence of selenolin on the metabolic functions of the liver of laboratory rats was studied on the model of the reproduction of acute liver failure caused by acetaminophen. It was determined that the use of selenolin inhibits and weakens the hepatotoxic effects, contributing to the correction of impaired homeostasis in rats. Under the action of the preparation, there was a decrease in enzymatic activity – AST – in 2.35 times, ALT – in 2.4 times, γ-GGT – by 21.7%, as well as an improvement in protein synthesizing function, manifested by an increase in the concentration of total protein by 26.9%. Selenolin significantly (p <0.05) reduces the level of lipid peroxidation products – DC – in 1.5 times, CD – by 56.3%, MDA – by 19.9%. Due to the organic selenium included in the preparation, selenolin helps to maintain a high activity of the enzyme antioxidant system, which leads to a decrease in the stationary level of free radical and lipid peroxidation products, allowing the body to quickly cope with pathological changes in the liver cells and in the blood homeostasis system.

The article is devoted to the study of the assessment of selenium status in the pathogenesis of liver diseases and the effectiveness of the use of a selenium-containing drug. In the course of studies in animals with clinical signs and high rates of hepatopathies markers – transaminases, in 100% of cases a low level of selenium was revealed – 0.026 ±0.01 μg / ml, because of the increased consumption of this microelement on the background of increased free radical oxidation processes in the liver tissue. It was found that intramuscular administration of the drug selenolin at a dose of 20 mg / 100 kg four times with an interval of 15 days significantly improved the clinical status of the experimental animals; the effectiveness of therapy was 93.3%. Parenteral administration of selenolin favorably corrected the processes of lipid peroxidation and contributed to a significant (p <0.01) decrease in the level of liver indicator enzymes in comparison with the control by 34.5% (aspartate aminotransferase) and by 45.8% (alanine aminotransferase), and also contributed to an increase in the level of selenium to the level of the physiological norm (0.068 ± 0.03 μg / ml).

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