Soybean is an important oilseed cum vegetable crop, susceptible to various biotic stresses which is attributed to recent decline in crop productivity. The emergence of virulent biotypes/strains of different plant pathogens necessitates the development of new crop varieties with enhanced host resistance mechanisms. Pyramiding of multiple disease-resistant genes is one of the strategies employed to develop durable disease-resistant cultivars to the prevailing and emerging biotypes of pathogens. The present study, reports the successful introgression of two major R-genes, including Rps2 (Phytophthora rot resistance), Rmd-c (complete-powdery mildew resistance) and effective nodulating gene (rj2) through functional Marker-Assisted Backcross Breeding (MABB) in the genetic background of well-adapted and high yielding soybean varieties, CO 3 and JS 335. We have identified several promising introgressed lines with enhanced resistance to Phytophthora rot and powdery mildew. The improved soybean lines have exhibited medium to high level of resistance against powdery mildew and Phytophthora rot as well as displayed effective nodulation capacity. Our study has proven the generation of resistant genotypes to realize the potential of MABB for achieving host plant resistance in soybean. the improved lines developed can greatly assist the soybean breeding programs in India and other soybean growing countries for evolving disease-resistant varieties. Phytophthora root and stem rot and powdery mildew are the widespread diseases of soybean crop affecting all major plant parts including seeds, stems, leaves, and roots. Many of the high yielding soybean mega-varieties are highly susceptible to both diseases 1-3. Infections by these pathogens cause severe tissue collapse in host plant that negatively impacts plant growth and productivity. Many of the chemical, cultural and biological methods suggested are practically inadequate to combat these diseases successfully 1,4,5. However, a sustainable approach for combating pathogenesis is to introgress the disease resistant (R-genes) and QTL from the identified soybean lines in to cultivars 5. Although many research groups have successfully introgressed R-genes into an elite variety 6,7 through marker-assisted breeding, there are very few reports of utilizing marker-assisted backcross breeding (MABB) method. Successful MABB was reported in soybean for mobilizing the null allele of β-conglycinin α-subunit 8 and Kunitz trypsin inhibitor 9. Following this example, it will be valuable to introgress the R-gene(s) through MABB to achieve high recurrent parent genome recovery (RPG) while eliminating the linkage-drag effect on target chromosome(s).

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