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The poly(A) (+)RNA populations from avocado fruit (Persea americana Mill cv. Hass) at four stages of ripening were isolated by two cycles of oligo-dT-cellulose chromatography and examined by invitro translation, using the rabbit reticulocyte lysate system, followed by two-dimensional gel electrophoresis (isoelectric focusing followed by sodium dodecyl sulfate polyacrylamide gel electrophoresis) of the resulting translation products. Three mRNAs increased dramatically with the climacteric rise in respiration and ethylene production. The molecular weights of the corresponding translation products from the ripening-related mRNAs are 80,000, 36,000, and 16,500. These results indicate that ripening may be linked to the expression of specific genes.

Flowering of the long day plant Hyoscyamus niger L., which is strictly photoperiodically controlled, was induced by 58 h continuous white light. The RNA from the leaves was isolated from photoperiodically induced and non‐induced plants and the poly(A)‐rich RNA separated by affinity chromatography on oligo‐dT‐cellulose. The poly(A)‐rich RNA was translated in vitro in the presence of 35S‐methionine using a rabbit reticulocyte lysate. Subsequent separation of the translation products by two‐dimensional polyacrylamide gel electrophoresis and fluorography allowed a comparison of the polypeptide pattern from induced and non‐induced leaf m‐RNA. The results indicate that induction of flowering is reflected by changes in the translation of several leaf polypeptides. These polypeptides were characterized by their isoelectric points and molecular masses.

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