Background & Aims: Given the importance and role of the organization's human resources, focusing and addressing issues such as the quality of work life that lead to increased employee performance, reduced absenteeism and leave of duty is important. Quality of work life is a set of organizational conditions and is created when the management of the organization is democratic, staff are treated with respect, and a safe work environment is provided. In other words, improving the quality of work life in staff is one of the important factors to ensure the sustainability of the health system. Considering the sensitive and stressful job situation of emergency center staff and the contradictory results of researches conducted on the quality of life of staff, the present study aimed to analyze the relationship between perceived organizational support and quality of work life of the operational staff of Tehran emergency centers. Materials & Methods: This descriptive, cross-sectional, correlational study was conducted on the operational staff of Tehran Emergency Center in the second six months of 2019. The sampling method was multi-stage stratified with the proportional allocation and 200 qualified staff from the six emergency centers of Tehran (north, south, east, west, center, Islamshahr) were included in the study. Inclusion criteria were having a diploma and higher degrees and having at least six months of experience as operational staff. Demographic information form, quality of work life questionnaire developed by Walton, and Eisenberg perceived organizational support questionnaire were used for data collection. The researcher returned 24-48 hours after the distribution of the questionnaires and collected the completed questionnaires. Then, the data were analyzed using independent t-test, ANOVA, Pearson's correlation-coefficient, and Kruskal-Wallis tests in SPSS version 16. Results:The results of the current study revealed that 42.2% and 41.1& of staff considered their income to be insufficient and somewhat sufficient respectively and the average work experience of the surveyed staff was 10.68 ± 6. The quality of work-life of staff of medical emergency center of Tehran was 96.1 ± 22 which was moderate. The quality of work life in the dimension of social integration in the job with an average of 3.09 ± 0.97 was the highest and in the dimension of the role of work life with an average of 2.35 ± 0.87 was the lowest among other