As a result of a series of experiments to study the entomopathogenic activity of bacteria and viruses from the “State Collection of Entomoacariphages and Microorganisms”, it was revealed that the large wax moth Galleria mellonella L. it is sensitive to new strains of bacteria and viruses. In the future, the strains can be used in the subsequent stages of screening of microorganisms that are promising as agents of biological pest control. The maximum biological efficacy on the fifth day against the tested insect was observed when using bacterial strains Bacillus spp. BZR 1159 (94.6%) and BZR 936 (95.0%) and a granulovirus strain of the codling moth (CpGV) BZR L-5 (100%). Larvae of G. mellonella L were susceptible to melanization in the process of infection with new bacterial and viral agents.

Possibility to apply wide range CO-laser for detection of trace gas pollutions in multicomponent gas mixture is studied both theoretically and experimentally. Parameters of sensitivity and selectivity for multicomponent gas analysis with CO-laser were calculated.

In laboratory conditions, a screening of 27 new aboriginal strains of bacteria from the Bioresource collection of the Federal State Budgetary Scientific Institution VNIIBZR "State collection of entomoacariphages and microorganisms" was carried out. As a result of the research, 6 new strains of bacteria with high and 2 strains with medium antagonistic activity were identified, which will be further considered for the next stage of research.

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Excessive application of chemical insecticides in agriculture has led to some serious problems that threaten the environment and human health. One of the possible ways to overcome the situation is to shift to environmentally-friendly preparations based on entomopathogenic bioagents. The purpose of this review was to search for and systematize methodological approaches to determine the entomopathogenic activity of microorganisms of various taxonomic groups in laboratory conditions. The expediency of choosing the necessary method when studying a particular phenomenon or process is an important point both in the fundamental and applied field of research. The primary assessment of the entomopathogenic activity of microorganisms in vitro is the basis for expanding the spectrum of their action, introducing new strains into collections. It also contributes to the replenishment of knowledge about the already known properties of various types of microorganisms. All this, certainly, is connected with the further choice of them as biological agents. Each of the presented groups of entomopathogens has its distinctive features of the mechanisms of action determined by the target insect. Concerning the choice of methodological approaches for assessing the entomopathogenic effect of fungi, the authors relied on the physiological characteristics of the insect and its stage of harmfulness. The researchers were guided by methodological approaches of oral infection of insects using an infected food source to study the activity of bacterial strains. Pathological changes in the cellular structure, as well as deformations of intestinal elements, were noted. The most common way to assess the effect of entomopathogenic viruses in the laboratory is the method of surface infection of the food source of the tested insect, taking into account the high specialization of the agent. When studying the mechanisms of action of fungal, bacterial and viral agents, researchers injected a suspension of the pathogen into the insect’s body. The search and systematization of relevant methodological approaches to assess entomopathogenic microorganisms depending on the taxonomic affiliation is an important part of the work directly related to the development of a high-quality and effective bioinsecticide.