Sweet potato is a valuable staple crop that guarantees food security to a large segment of the world population. The wide phenotypic and genetic variability of this species is an indication of its high adaptation capacity to diverse environmental conditions. In Colombia, it is a neglected and underutilized crop, mainly managed by traditional knowledge. The aim of this study was to recognize the contribution of in situ conservation and to characterize the habitats and the traditional uses to shed light on the design of their management and conservation strategies. Germplasm and data collection were conducted in the Caribbean and Andean regions of the country. This collection resulted in 750 accessions from 131 municipalities, belonging to 19 departments of the two regions. In these regions, sweet potato has been conserved in situ in a wide spatial and altitudinal distribution. The major collection sources were wild and cultivated habitats, which highlight the invaluable contribution of farmers and communities in the preservation of this species and its associated knowledge. In situ conservation seemed to be an efficient strategy for conserving and using plant genetic resources; therefore, it should be considered by conservation efforts. [germplasm collection, habitats, in situ conservation, Ipomoea batatas]