We present the complete genome sequence for a novel Edwardsiella ictaluri-specific bacteriophage, PEi21, isolated from river water in Japan. An initial comparative genome analysis revealed that the phage was closely related to the previously reported Edwardsiella tarda phage MSW-3 isolated from a red sea bream farm in Japan.
E d wa r d s i e l l a i c t a l u r i , a me mb e r o f t h e f a mi l y E n t e r o b a c t e r i a c e a e , i s t h e c a u s a t i v e a g e n t o f e n t e r i c s e p t i c e mi a o f c a t f i s h ( ESC) 1 ) wh i c h i s r e c o g n i z e d a s o n e o f t h e mo s t s i g n i f i c a n t c a u s e s o f mo r b i d i t y a n d mo rt a l i t y i n c u l t u r e d c h a n n e l c a t f i s h I c t a l u r u s p u n c t a t u s i n s o u t h e a s t e r n Un i t e d S t a t e s 2 ). A l t h o u g h E . i c t a l u r i i n f e ct i o n wa s r e p o r t e d i n r a i n b o w t r o u t On c o r h y n c h u s my k i s s a n d a f e w o r n a me n t a l f i s h s p e c i e s , E . i c t a l u r i i s c o n s i d e r e d p r i ma r i l y t h e p a t h o g e n o f c a t f i s h . I n a d d it i o n t o c h a n n e l c a t f i s h i n t h e US A , t h e d i s e a s e h a s b e e n r e p o r t e d i n v a r i o u s c a t f i s h s p e c i e s i n c l u d i n g b r o wn b u l lh e a d A me i u r u s n e b u l o s u s , wh i t e c a t f i s h A . c a t u s , b l u e c a t f i s h I . f u r c a t u s , wa l k i n g c a t f i s h Cl a r i a s b a t r a c h u s , s t r i p e d c a t f i s h P a n g a s i u s h y p o p h t h a l mu s , a n d y e l l o w c a t f i s h P e l t e o b a g r u s f u l v i d r a c o , i n t h e US A a n d A s i a n c o u n t r i e s 2 -4 ) .
E . t a r d a , a n o t h e r s p e c i e s i n t h e g e n u s E d wa r d s i e l l a , i s we l l k n o wn a s t h e c a u s a t i v e a g e n t o f e d wa r d s i e l l o s i s i n v a r i o u s c u l t u r e d f r e s hwa t e r a n d ma r i n e f i s h s p e c i e s i n J a p a n a s we l l a s o t h e r c o u n t r i e s 2 , 5 , 6 ) , b u t t h e r e h a v e b e e n n o r e p o r t s o n E . i c t a l u r i i n J a p a n . I n 2 0 0 7 , h o we v e r , E . i c t a l u r i wa s f i r s t i s o l a t e d f r o m d i s e a s e d a y u i n t h e r i v e r s o f T o k y o Me t r o p o l i s , Y a ma g u c h i P r e f e c t u r e a n d Hi r o s h i ma P r e f e c t u r e 7 ), a n d t h e n t h e i n f e c t i o n wa s r e c o r d e d i n ma n y r i v e r s o f we s t e r n J a p a n i n 2 0 0 8 a n d 2 0 0 9 ( p e r s o n a l c o mmu n i c a t i o n wi t h J a p a n F i s h e r i e s Re s o u r c e Co n s e r v at i o n A s s o c i a t i o n ) . T h e E . i c t a l u r i i s o l a t e s f r o m a y u i n v a r i o u s l o c a t i o n s we r e h i g h l y h o mog e n e o u s i n t h e c o n v e n t i o n a l b i o c h e mi c a l a n d g e n e t i c c h a r a c t e r i s t i c s 8 ) . I n t h i s s t u d y , we e x a mi n e d s e r o l o g ic a l l y t h e E . i c t a l u r i i s o l a t e s f r o m a y u b y a g g l u t i n a t i o n wi t h r a b b it a n t i s e r a .
Ma t e r i a l s a n d Me t h o d s
Ba c t e r i a l s t r a i n s T h i r t e e n s t r a i n s i s o l a t e d f r o m wi l d a y u a t d i f f e r e n t a r e a s i n J a p a n i n 2 0 0 7 a n d 2 0 0 8 we r e u s e d i n t h i s s t u d y . T h e f o u r s t r a i n s i n 2 0 0 7 we r ...
E d wa r d s i e l l a i c t a l u r i は従来ナマズの病原体とされてきたが(Ha wk e e t a l . , 1 9 8 1 ; E v a n c e e t a l . , 2 0 1 1 ) ,本菌に よる感染症が 2 0 0 7年の 8月から 1 0月にかけて,東京 アユ死亡魚から分離された PH-0 7 4 4 (Sa k a i e t a l . , 2 0 0 8 ) ,アユ保菌魚から分離された A H0 8 1 6 (Ha s s a n e t
都,山口県および広島県下の河川アユ Pl e c o g l o s s u s a l t i v e l i sに発生して一部大量死をもたらした(S
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.