Penelitian ini bertujuan untuk mengetahui pengaruh dan dosis yang terbaik pada pemanfaatan diatomae Chaetoceros ceratosporum dalam formula pakan terhadap peningkatan respon imun seluler udang windu (Penaeus monodon Fab.). Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan 3 ulangan. Sebagai perlakuan adalah pemanfaatan Chaetoceros ceratosporum dalam formula pakan iso protein 39,02% dan iso energy 3,58 kkal g pakan dengan dosis yang berbeda yaitu: A (0%), B (3,04%), C (6,08%) dan D (9,12%). Parameter yang diamati adalah total hemosit, total diferensial hemosit (hyaline, semi granular dan granular), dan aktivitas vibriocidal. Berdasarkan hasil penelitian menunjukkan bahwa pemanfaatan diatomae Chaetoceros ceratosporum dalam formula pakan dapat meningkatkan respon imun seluler, dan dosis terbaik berkisar 5,15%-6,51%.
Infectious disease is still a health problem in the world, especially in tropical and developing countries, including Indonesia. The diagnosis of tropical disease cases mostly are still based on the main of clinical symptoms, that is fever. Among 900 fever patients, 21% of the disease were diagnosed by clinical examinations, 35% by laboratory tests and 40% were diagnosed as fever of unknown origin (FUO). One forth of the FUO patients were definitively diagnosed after specific laboratory tests, but the use of the specific tests in daily practice were relatively rare. The tests ordered by physicians to the Department of Central Clinical Laboratory Dr. Saiful Anwar Hospital Malang, are mostly hematology and blood chemical tests (59.54% and 21.52%). Specific tests for microbiology and parasitology were only 3.15% and 0.46% respectively. Fever patients who not definitively diagnosed, often received unnecessary treatments or medications (over-treatment), those are actually dangerous to them. To improve the quality of clinical management, the traditional laboratory service should be changed to be the interactive laboratory service. It needs a role, understanding and good collaboration between clinical practitioners who know the basic sciences and the pre/para-clinical scientists who interest to the clinical phenomenon. The aim of the change is to get the more scientific, ethical and professional medical services.Key words: Laboratory medicine, diagnosis,management, infectious disease 1. PENDAHULUAN Latar BelakangPenyakit infeksi sampai saat ini masih menjadi masalah kesehatan utama di dunia. Hal ini disebabkan karena penyakit yang disebabkan oleh infeksi berbagai jenis bakteri, virus, jamur maupun parasit masih merupakan penyebab kesakitan dan kematian tertinggi, terutama di negara-negara tropik. Masalah ini menjadi semakin komplek dengan timbulnya berbagai jenis penyakit infeksi yang termasuk : "The New Emerging and Reemerging Infectious Diseases". Ternyata kelompok penyakit ini telah menjadi perhatian khusus di berbagai negara di dunia, dan sempat menghantui negara-negara maju, seperti Jepang, USA, Australia dan negara-negara di benua Eropah. Demikan juga halnya di Indonesia. Beberapa penyakit yang telah dikenal sejak lama dan masih tetap menjadi masalah seperti tuberkulosis, malaria dan demam berdarah, akhir-akhir ini menunjukkan kecenderungan peningkatan angka kesakitan maupun kematian. Di sisi lain, berbagai penyakit yang secara klinis hanya menunjukkan gejala ringan dan dianggap tidak mengancam jiwa (non life threatening) seperti malaria tanpa penyulit, toxoplasmosis dan infestasi cacing, sering kali tidak terdiagnosis, bahkan terlepas dari perhatian kita sehingga tidak tertangani dengan baik. Sebagai contoh, di berbagai daerah Indonesia di luar Jakarta, angka kejadian infestasi cacing usus masih berkisar antara 40% -60% untuk semua golongan umur dan 60% -90% di kalangan anak-anak sekolah (1,2), namun demikian penanggulangan terhadap penyakit kecacingan terkesan seolah diabaikan, padahal hal ini sangat be...
This experiment aims to determine the effect and the best dose of Chaetoceros ceratosporum diatomae utilization in feed formula for post larvae of tiger shrimp (Penaeus monodon Fab.) growth and immunity to Vibrio harveyi infection. This research applied Completely Randomized Design (CRD) with 4 treatments and 3 replications. The treatment use Chaetoceros ceratosporum diatomae in feed formula (iso protein 39.02% and iso energy 3.58 kcal/g diet) in different doses, i.e. treatment A = 0 %; B = 3.04 %; C = 6.0 8%; D = 9.12 %. Observed parameters were Survival Rate, Growth Rate, Food Conversion Ratio (FCR) and Protein Efficiency Ratio (PER). Result showed that Chaetoceros ceratosporum diatomae utilization in feed formula affect the increase of growth and immunity of post larvae of tiger shrimp (Penaeus monodon Fab.) to Vibrio harveyi infection. The best dose in feed formula ranged from 5,75%-5,95%.
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