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Sleep plays an essential role in maintaining employees’ health and well-being. However, stressors, such as conflict at work, may interfere with employees’ sleep. Drawing on previous literature on the relationship between conflict at work and sleep outcomes, we proposed a negative relationship between daily conflict at work and physiological changes during early sleep, particularly nocturnal heart rate variability (HRV). Furthermore, building on the perseverative cognition hypothesis, we proposed that daily work-related rumination mediates the relationship between conflict at work and nocturnal HRV. Ninety-three healthcare employees participated in a daily diary study for five workdays, resulting in 419 observations. Multilevel analysis revealed a significant relationship between daily conflict at work and nocturnal HRV, specifically high-frequency (HF) power. Daily conflict at work was found to predict rumination; however, rumination did not significantly predict nocturnal HRV. Our results suggest that daily conflict at work increases rumination during the off-job time and may directly alter nocturnal HRV, specifically parasympathetic function in early sleep.

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