Objective. The present study aimed to compare variations in quantified tumor necrosis factor-alpha (TNF-α) levels in patients with periodontitis stage 2 grade B (POD2B) and/or type 2 diabetes (T2D) and to identify any relationships between this cytokine and these diseases. Methods. Levels of the cytokine TNF-α in gingival crevicular fluid in patients with POD2B and/or T2D were evaluated. A total of 160 subjects were distributed into four groups: those with POD2B (n=44); those with T2D (n=37); those with POD2B/T2D (n=40); and healthy subjects (n=39). Glycosylated hemoglobin (HbA1c) and blood glucose (BG) levels were quantified in each subject. Data were collected on body mass index (BMI), loss of insertion (LI), and probe depth (PD). Gingival crevicular fluid samples were collected from the most acutely affected periodontal pocket and gingival sulcus in each subject, and TNF-α was quantified by multiplex analysis. Results. Kruskal Wallis tests was used to identify differences in TNF-α levels, LI, PD, BMI, BG, and HbA1c by group. Differences (p<0.001) were found for LI, PD, BG, and HbA1c. A Spearman test was used to calculate possible correlations between TNF-α levels and LI or PD identified a weak but significant negative correlation of TNF-α with LI (Rho=-0199; p=0.012), and a moderately positive correlation of LI with PD (Rho=0.509; p < 0.001). Conclusions. No variation was found between TNF-α levels and the presence of POD2B, POD2B/T2D, or T2D, suggesting the absence of any direct relationship between progression of these diseases and TNF-α levels. However, a correlation was present between low TNF-α concentrations and greater LI.

Objective. The present study aimed to compare levels of matrix metalloproteinase-9 (MMP-9) and myeloperoxidase (MPO) in gingival crevicular fluid (GCF) from subjects with controlled and noncontrolled Type 2 Diabetes Mellitus (T2D), with and without stage 2 grade B periodontitis (POD2B) versus healthy (H) subjects. Methods. The levels of both enzymes, from 80 GCF samples collected with PerioPaper strips, were analyzed by a Multiplex/Luminex assay. Five groups were formed, all current patients at the Institutional Dentistry Service, and distributed as follows: two groups of diabetics (one controlled and one poorly controlled); two groups with the previous conditions and diagnosed with POD2B; and one H group. Results. The highest concentration of MMP-9 corresponded to the H group, while the lowest corresponded to the T2D controlled group. Regarding MPO levels, the highest levels were associated with the T2D controlled with POD2B group and the lowest with the T2D controlled group. Conclusions. No apparent relationship between the elevation of MMP-9 and MPO levels was observed among subjects with T2D, with and without POD2B, compared to H subjects.

Antecedentes. Entre los factores de riesgo para periodontitis están descritos los genéticos, siendo la interleucina 6 (IL-6) una citocina implicada en el proceso inflamatorio y destrucción periodontal. Los polimorfismos en el gen IL-6 pueden explicar el riesgo de padecer la enfermedad en ciertas poblaciones.Objetivo. Determinar la asociación del polimorfismo g.-174G>C del gen IL-6 con la periodontitis Estadio II grado B.Material y métodos. Estudio descriptivo, transversal, prospectivo y de casos y controles. Se analizaron las frecuencias genotípicas y alélicas de g.-174G>C, en 102 sujetos (52 casos periodontitis Estadio II grado B y 50 controles, mediante la técnica de PCR cuantitativa (qPCR). Se determinó la asociación del polimorfismo con el riesgo para periodontitis, mediante análisis univariado. Resultados. Se determinaron las frecuencias genotípicas para el polimorfismo g.-174 G>C. El genotipo homocigoto silvestre GG fue el más prevalente (90.4%) en los casos y en los controles (78%), al igual que el alelo G 95.2% y 88%, respectivamente. El genotipo homocigoto mutado CC se observó disminuido en los controles y ausente en los casos. El análisis no mostró asociación del polimorfismo g.-174 G/C con la periodontitis Estadio II grado B.Conclusión. No se observó asociación entre el polimorfismo g.-174 G/C y la periodontitis Estadio II grado B. Asimismo, se ha reportado una baja frecuencia del genotipo homocigoto mutado CC, el cual se encontró en el 2% de los controles y ausente en los casos estudiados.

This study aimed to evaluate the effectiveness of using an ionized monocalcium phosphate and enamelin derivatives (IMP+ED) based mouthwash for the treatment of dentin hypersensitivity (DH) after scaling and root planing (SRP). 47 patients who reported DH after SRP treatment were included in this prospective cohort study. The Schiff Cold Air Sensitivity Scale (SCASS) was applied to classify their degree of pain in mild, moderate or intense at two times: after SRP (T0), and after one month of using a IMP+ED-based mouthwash (T1). The McNemar-Bowker test was used to compare the correlated proportions between both times (p<0.05). After the SRP therapy (T0), all the sample members reported pain distributed in the following manner: 12.8% were mild, 27.6% moderate, and 59.6% intense. At one month since treatment and with the use of the IMP+ED-based mouthwash (T1), the distribution of pain levels changed to 83% mild, 12.8% moderate, and 4.3% intense, this change was statistically significant (p<0.001). IMP+ED-based mouthwash produces a positive effect in reducing painful responses caused by exposure of the dentin tubules to the oral environment after SRP therapy.