We demonstrate a novel ultrafast Multifocus 25-camera-array microscope (M25) for truly simultaneous high-resolution 3D imaging of 25 focal planes. Customized for functional neural circuit imaging, our M25 microscope captures 130× 130× 50um3 volumes at >100Hz.

We demonstrate an ultrafast Multifocus 25-camera-array microscope (M25) for truly simultaneous 3D imaging of 25 focal planes of a living specimen. Customized for functional neural circuit imaging, our M25 prototype captures 130x130x50um3 volumes at >100Hz.

We have designed and constructed a 3D microscope that combines Multifocus Microscopy with Structured Illumination Microscopy for live 3D super-resolution biological imaging. Imaging multiple focal planes simultaneously requires a new approach to image reconstruction.