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Summary ± ZusammenfassungAmino sugars make a significant contribution to soil organic N and are mainly of microbial origin. The most important amino sugars in soil are glucosamine, galactosamine, muramic acid, and mannosamine. A method was developed for the simultaneous determination of these four amino sugars by high-performance reverse-phase liquid chromatography in standard solutions, soil and root hydrolysates. Pre-column derivatization with o-phthaldialdehyde (OPA) was used in an automated sample injector with thermostatic regulation of the reagent at 4 C. The separation of the four amino sugars was fully satisfactory and was not disturbed by other fluorescent components in the soil and root hydrolysates.

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