Papaya plant (Carica papaya L) is also called medicinal plant which is used in traditional medicine. Part of papaya plants (Carica papaya L) which often utilized are papaya leaf (Carica papaya L) because its content of substances or active ingredients is antibacterial, cancer prevention, increase appetite, and treat some diseases caused by bacteria. The aim of this research was to determined the extracted ethanol of papaya leaf (Carica Papaya L) with the growth of Escherichia coli bacteria by the diffusion method. Research design which used in this study was experimental research, with the method of sampling purposive sampling, then the results analyzed with Friedman Test. A result of research from 5 replications test of extract concentration of papaya leaf by diffusion method obtained that the minimum average concentration was 10,00 and maximize was 14,20. While the minimum inhibitory zone diameter was 10 mm with the concentration of 10% and the maximum was 15 mm with the concentration of 20%. A result of Friedman test, it was found that (p = 0,00 <0,05) so it can be declared that there was a difference of papaya leaf extract (Carica Papaya L) effect on Escherichia coli bacteria growth by the diffusion method.Abstrak: Tanaman pepaya (Carica papaya L) disebut juga tanaman obat yang bermanfaat untuk pengobatan tradisional. Bagian yang sering dimanfaatkan pada tanaman pepaya (Carica papaya L) adalah daun pepaya (Carica papaya L) karena mengandung zat atau bahan aktif yang bersifat sebagai antibakteri, pencegahan kanker, menambah nafsu makan dan mengobati beberapa penyakit yang disebabkan oleb bakteri. Tujuan penelitian adalah untuk mengetahui ekstrak ethanol daun pepaya (Carica Papaya L) terhadap pertumbuhan bakteri Escherichia coli dengan metode difusi. Desain penelitian yang digunakan dalam penelitian ini adalah penelitian eksperimental dengan metode pengambilan sampel purposive sampling, selanjutnya hasil dianalisis menggunakan uji Friedman. Hasil penelitian dari 5 penggulangan uji konsentrasi ekstrak daun pepaya dengan metode difusi dapat diketahui rata-rata konsentrasi minimum 10,00 dan maksimun 14,20. Sedangkan diameter zona hambat minimum adalah 10 mm dengan konsentrasi 10% dan maksimun adalah 15 mm dengan konsentrasi 20%. Hasil Friedmen test diperoleh nilai (p = 0,00 < 0,05) sehingga dapat dinyatakan bahwa terdapat perbedaan efektiftas ekstrak daun pepaya (Carica Papaya L) terhadap pertumbuhan bakteri Escherichia coli dengan metode difusi.
International Commite for Standarization in Hematology (ICSH) recommends hemoglobin examination using Cyanmethemoglobin method. Examination of Hb Cyanmethemoglobin using sahli pipette in sampling. The Sampling method flow through the hose. The use of glass pipets do not have high accuracy and precission for less than volumes 1 ml, so in small fluid displacement less than 1000 (microliter) using micropipet or commonly known as automatic pipette. The aim of this research was to know the precision value between sampling using the sahli pipette and mikropipet on examination of hemoglobin value of Cyanmethemoglobin method accepted or not on standard Clinical Laboratoty Improvement Amendments (CLIA) and to analyze the difference of precision between sampling using pipette sahli and micropipette on examination of hemoglobin Cyanmethemoglobin method. The design of this study was descriptive anlytic. This research using EDTA blood examination with the number of 36 test samples. Preliminary period expression used 60 test samples. The hemoglobin examination using Cyanmethemoglobin method by spectrophotometry. Based on the results of the research, the value of % CV of sahli’s pipet is 4.60% and the value % CV of micropipet is 2.02%. and the value %CV The maximum recommended by the CLIA can be received from the sahli and mikropipet pipette that is <7%. he data obtained were statistically analyze by using Willcoxon Test and the result P 0.000 <0.05. The conclution of this research was found a signifcant difference between Sahli pipette and micropipette.Abstrak: International Commette for Standarization in Hematology (ICSH) merekomendasikan pemeriksaan hemoglobin dengan menggunakan metode Cyanmethemoglobin. Pemeriksaan Hb Cyanmethemoglobin menggunakan pipet sahli dalam pengambilan sampel. Pengambilan sampel dengan cara dihisap melalui selang. Penggunaan pipet gelas tidak mempunyai akurasi dan presisi yang tinggi untuk volume kurang dari 1 ml, sehingga pemindahan cairan dengan volume kecil kurang dari 1000 (microliter) orang cenderung menggunakan mikropipet atau biasa juga dikenal dengan pipet otomatis. Penelitian ini bertujuan untuk mengetahui nilai presisi antara pemipetan sampel menggunakan pipet sahli dan mikropipet pada pemeriksaan kadar hemoglobin metode Cyanmethemoglobin diterima atau tidak pada standar Standar Clinical Laboratoty Improvement Amendments (CLIA) dan menganalisis perbedaan presisi antara pemipetan sampel menggunakan pipet sahli dengan pemipetan sampel menggunakan mikropipet pada pemeriksaan kadar hemoglobin metode Cyanmethemoglobin. Desain penelitian ini adalah deskriptif analitik. Penelitian ini menggunakan bahan pemeriksaan berupa darah EDTA dengan jumlah 36 sampel uji. Ditahapan periode pendahuluan digunakan sebanyak 60 sampel uji. Metode pemeriksaan hemoglobin menggunakan metode Cyanmethemoglobin secara fotometri. Berdasarkan hasil penelitian yang dilakukan diperoleh nilai %CV pipet sahli sebesar 4,60% dan nilai %CV mikropipet sebesar 2,02%. Nilai %CVmaksismum yang di rekomendasikan oleh CLIA dapat diterima dari pipet sahli dan mikropipet yaitu <7%. Data yang diperoleh diolah secara statistik menggunakan uji Willcoxon didapatkan hasil P 0.000<0.05. Sehingga dapat disimpulkan ada perbedaan signifkan antara pipet sahli dan mikropipet.
Pandanus leaf is one of the plants that contain active substances that can function as antibacterial. Active substances contained in pandan leaves can be used as traditional medicine in curing various diseases. This study was aimed to determine the effectiveness of boiled water of pandan leaves (Pandanus amaryllifolius) in inhibiting the growth of Salmonella typhi bacteria with the Dilution Test Method. The research design used was quasi-experiment with sampling technique used in this research was purposive sampling. Handling the sample by boiling then boiled water was diluted according to the desired concentration. The research was conducted at the Bacteriology Laboratory of Medical Laboratory of Health Ministry of Health Polytechnic Pontianak on February-April 2013. Then, data was processed by Friedman statistic test. Based on the research results of the effectiveness of boiled water of pandan leaves (Pandanus amaryllifolius) in inhibiting the growth of Salmonella typhi bacteria with Dilution Test Method can be concluded that the minimum inhibitory content of boiling water of pandan leaves effectively inhibits Salmonella typhi bacteria growth at concentration 60%. The statistical results show p-value of 0.000 (p = 0,000 <0,05), it can be concluded that pandan leaves boiling water is effective in inhibiting the growth of Salmonella typhi bacteria with dilution test method. Abstrak: Daun pandan merupakan salah satu tanaman yang mengandung zat-zat aktif yang dapat berfungsi sebagai antibakteri. Zat aktif yang terkandung pada daun pandan dapat digunakan sebagai obat tradisional dalam menyembuhkan berbagai macam penyakit. Penelitian ini bertujuan untuk mengetahui efektiftas air rebusan daun pandan (Pandanus amaryllifolius) dalam menghambat pertumbuhan bakteri Salmonella typhi dengan Metode Dillution Test. Desain penelitian yang digunakan adalah eksperimen semu dengan teknik pengambilan purposive sampling. Penanganan sampel dengan cara direbus kemudian air rebusan tersebut dilakukan pengenceran sesuai konsentrasi yang diinginkan. Penelitian dilakukan di Laboratorium Bakteriologi Jurusan Analis Kesehatan Poltekkes Kemenkes Pontianak pada bulan Februari - April 2013. Selanjutnya data diolah dengan Uji statistik Friedman. Berdasarkan hasil penelitian dapat disimpulkan bahwa kadar hambat minimum air rebusan daun pandan yang efektif menghambat pertumbuhan bakteri Salmonella typhi yaitu pada konsentrasi 60 %. Hasil statistik menunjukkan nilai p sebesar 0,000 (p = 0,000 < 0,05), maka dapat disimpulkan bahwa air rebusan daun pandan efektif dalam menghambat pertumbuhan bakteri Salmonella typhi dengan metode dillution test.
Acorus sp (red sweet flag) is an endemic plant from West Borneo which contains chemical substances such as flavonoids that have bioactivity as antioxidants so it is recommended one to prevent cancer. The study aimed to determine toxicity of n-Hexan extract and methanol red sweet flag rhizomes against Artemia salina Leach with Brine Shrimp Lethality Test method. This study was a type of quasi experimental design, sampling technique obtained by using purposive sampling. According to the study results, it can be seen than methanol extract from red sweet flag rhizomes give impact on the mortality average of Artemia salina Leach : concentration 100 µg/ml (51%), 250 µg/ml (75%), 500 µg/ml (92%), 750 µg/ml (96%) and 1000 µg/ml (99%). In addition, n-Hexan extract of red sweet flag rhizomes in 100 µg/ml (45%), 250 µg/ml (60%), 500 µg/ml (92%), 750 µg/ml (84%) and 1000 µg/ml (96%). It is reported based on probit analysis that LC50 of n-Hexan extract from red sweet flag rhizomes was 128.2921 µg/ml meanwhile methanol extract was 242.7169 µg/ml µg/ ml, thus in conclusion n-Hexan and methanol extract of red sweet flag rhizomes potentially cytotoxic to Artemia salina Leach larvae.Abstrak: Jeringau merah (Acorus sp) merupakan satu jenis tanaman endemik Kalimantan Barat memiliki kandungan kimia diantaranya flavonoidyang mempunyai bioaktivitas sebagai antioksidan sehingga sangat baik untuk pencegahan kanker. Penelitian ini bertujuan mengetahui mengetahui dan menentukan toksisitas ekstrak n-Heksan dan Methanol Rimpang Jeringau MerahterhadapArtemia salinaLeachdengan metode Brine Shrimp Lethality Test. Desain penelitian ini adalah eksperimental semu, teknik pengambilan sampel dengan cara purposive sampling. Berdasarkan hasil penelitian diketahui ekstrak Methanol Rimpang Jeringau Merah rerata kematianArtemia salina Leach konsentrasi 100 µg/ml (51%), 250 µg/ ml (75%), 500 µg/ml (92%), 750 µg/ml (96%) dan 1000 µg/ml (99%)pada ekstrak n-Heksan Rimpang Jeringau Merah konsentrasi 100 µg/ml (45%), 250 µg/ml (60%), 500 µg/ml (92%), 750 µg/ml (84%) dan 1000 µg/ml (96%). Dari analisis probit diketahuinilai LC50 ekstrak n-Heksan Rimpang Jeringau Merah yaitu 128.2921 µg/ml dan ekstrak Methanol Rimpang Jeringau Merah yaitu 242.7169 µg/ml µg/ml, maka disimpulkan ekstrak n-Heksan dan Methanol Rimpang Jeringau Merahbersifat sitotoksik terhadap larva Artemia salina Leach.
Red Jeringau (Acorus calamus L.) is one of the endemic plants of West Kalimantan which contains antibacterial, phytochemical, and antioxidant activity. This plant has been used for generations by people who live in the interior and away from health services as a mixture of traditional medicines. This study aims to determine the effect of the concentration of Jeringau Red rhizome water on the sensitivity of Escherichia coli, Salmonella typhi and Shigella sp with concentrations of 100%, 75%, 50%, and 25%. The Red Jeringau Rhizome is obtained from the Landak Regency and then boiled using distilled water. This study uses a quasi-experimental design with sampling techniques using purposive sampling. This study was conducted with 6 replications for each treatment. The effectiveness test of the Red Jeringau rhizome boiled water was carried out using the Kirby Bauer method with bacterial suspension which was adjusted to Mc Farland’s turbidity standard. The data obtained were then analyzed using a simple linear regression test and obtained p (0.00) <α (0.05) against the bacteria Escherichia coli, Salmonella typhi, and Shigella sp. This means that H1 is accepted so that it can be stated that there is an influence of the concentration of Jeringau Red rhizome water on Escherichia coli, Salmonella typhi, and Shigella sp. The magnitude of the contribution effect of Jeringau Red rhizome on bacterial sensitivity is 85.7% (R = 0.857) in Escherichia coli, 91.1% (R = 0.911) in Salmonella typhi, and 85.7% (R = 0.857) in Shigella sp.Abstrak Jeringau Merah (Acorus calamus L.) merupakan salah satu tanaman endemik Kalimantan Barat yang mengandung antibakteri, ftokimia, dan aktivitas antioksidan. Tumbuhan ini secara turun temurun dimanfaatkan masyarakat yang tinggal dipedalaman dan jauh dari pelayanan kesehatan sebagai ramuan obat tradisional.Penelitian ini bertujuan mengetahui pengaruh konsentrasi air rebusan rimpang Jeringau Merah terhadap sensitiftas bakteri Escherichia coli, Salmonella typi dan Shigella sp dengan konsentrasi 100%, 75%, 50% dan 25%. Rimpang Jeringau Merah diperoleh dari Kabupaten Landak kemudian direbus menggunakan aquadest. Penelitian ini menggunakan desain eksperimen semu (Quasi Experiment) dengan teknik pengmbilan sampel dengan cara purposive sampling. Penelitian ini dilakukan dengan 6 replikasi untuk masing-masing perlakuan. Uji efektiftas air rebusan rimpang Jeringau Merah dilakukan menggunakan metode Kirby Bauer dengan suspensi bakteri yang disesuaikan dengan standar kekeruhan Mc Farland. Data yang diperoleh kemudian dianalisis menggunakan uji regresi linear sederhana dan didapatkan p (0,00) < α (0,05) terhadap bakteri Escherichia coli, Salmonella typhi, dan Shigella sp. Hal ini berarti H1 diterima sehingga dapat dinyatakan bahwa terdapat pengaruh konsentrasi air rebusan rimpang Jeringau Merah terhadap bakteri Escherichia coli, Salmonella typhi, dan Shigella sp. Besarnya kontribusi pengaruh rebusan rimpang Jeringau Merah terhadap sensitiftas bakteri yaitu 85,7% (R=0,857) pada Escherichia coli, 91,1% (R=0,911) pada Salmonella typhi, dan 85,7% (R=0,857) pada Shigella sp.
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