The right hand end Nde I fragment 3 (90.8-100 map units) of the fowl adenovirus serotype 10 (FAV-10) was characterised so as to allow the location of an insertion site for recombinant vector construction. Infectious bursal disease virus (IBDV) VP2 gene from the Australian classical strain 002/73, under the control of the FAV-10 major late promoter/leader sequence (MLP/LS) was inserted into a unique Not I site that was generated at 99.5 map units. This recombinant virus was produced without deletion of any portion of the FAV-10 genome. When administered to specific pathogen free (SPF) chickens intravenously, intraperitoneally, subcutaneously or intramuscularly, it was shown that the FAV-10/VP2 recombinant induced a serum VP2 antibody response and protected chickens against challenge with IBDV V877, an intermediate virulent classical strain. Birds were not protected when the recombinant was delivered via the conjunctival sac.
The nucleotide sequence and genomic location of the fowl adenovirus serotype 10 (FAV-10) putative 33K and precursor protein VIII (pVIII) genes have been determined. The total genomic region sequenced was 1814 base pairs (bp) in length with the 33K coding region occupying the sequence from nucleotides 44 to 634 and the pVIII coding region nucleotides 949 to 1689. The location of both the 33K and pVIII genes have the same positional organization as their human adenovirus (HAV) counterparts which is 3 prime (3') to the 100K gene. Along with the 100K the 33K and pVIII form the late transcription unit 4 (L4). The FAV-10 putative 33K coding region could encode a polypeptide of 196 amino acids in length with a relative molecular mass of 21.9 kilodaltons (kDa) while the pVIII produces a polypeptide of 246 amino acids with a calculated relative mass of 26.7 kDa. Two possible splice acceptor sites were identified one 5' to the 33K and one 5' to the pVIII coding regions. A putative poly A recognition sequence of AATAAA was identified 3' to the pVIII, signaling the end of the L4 transcription unit.
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