Ganoderma lucidum (G. lucidum) has been widely used for its health benefits as an edible and traditional medicinal mushroom for thousands of years in Asian countries. It is currently used as a nutraceutical and functional food owing to its major bioactive compounds, polysaccharides and triterpenoids. G. lucidum exhibits a broad range of hepatoprotective impacts in various liver disorders, such as hepatic cancer, nonalcoholic fatty liver disease (NAFLD), alcohol-induced liver disease, hepatitis B, hepatic fibrosis, and liver injury induced by carbon tetrachloride (CCl4) and α-amanitin. G. lucidum protects the liver through a broad range of mechanisms that include the modulation of liver Phase I and II enzymes, the suppression of β-glucuronidase, antifibrotic and antiviral actions, the regulation of the production of nitric oxide (NO), the maintenance of hepatocellular calcium homeostasis, immunomodulatory activity, and scavenging free radicals. G. lucidum could signify an encouraging approach for the management of various chronic hepatopathies, and its potential mechanisms make it a distinctive agent when used alone or with other drugs and applied as a functional food, nutraceutical supplement, or adjuvant to modern medicine. This review summarizes the hepatoprotective properties of G. lucidum with its various mechanisms of action on different liver ailments. Biologically active substances derived from G. lucidum are still being studied for their potential benefits in treating different liver ailments.

BACKGROUND: The Lewis (b) blood group antigen-Binding Adhesion2 (BabA2) has been reported to mediate the attachment of H. pylori to human. AIM: assessment the diagnostic potential of detection of (BabA2) gene compared with immunostaining of Lewis (b) by specific mouse monoclonal antibodies in gastric biopsies from Egyptian Patients as a diagnostic maker for Helicobacter pylori infection. MATERIALS AND METHODS: Fifty untreated patients suffering from dyspeptic complaints were enrolled in this study and underwent for upper gastro-duodenal endoscopy. Biopsies were taken for histological examination by (H&E) and immunohistochemical analysis for Lewis b by specific mouse monoclonal antibodies, and scoring of Lewis b expression in gastric tissue biopsy as well as molecular detection of BabA2 gene of H. pylori by PCR. Biochemical analysis was performed to detect the presence of H. pylori urease activity using Rapid Urease Test (RUT). RESULTS : Out of 50 gastric biopsies, 41 biopsies were positive for histological, Immunostaining for Lewis b expression and urease activity test (RUT) for H pylori. RUT showed a sensitivity of 87.8%, specificity 88.9%, positive predictive value (PPV) 97.2%, and negative predictive value (NPV) 61.5%. BabA2 gene results revealed that, out of 41 positive biopsied cases, 39 (95.1%) were positive by the PCR test for BabA2 gene. And all 9 negative biopsies (100%) for H pylori negative for BabA2gene so the sensitivity and specificity of BabA2 gene detection in gastric biopsies by PCR were 95.1% and 100%; respectively. CONCLUSION : BabA2 gene detection in gastric tissue biopsies could be suggested as a diagnostic biomarker to be included among the other biomarkers routinely performed for clinical diagnosis of H. pylori infection.

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A b s t r a c t A r t i c l e I n f oEnergy drinking among college students is widespread and known to cause significant harms and hazards for the drinker. In this study we have determined the prevalence estimate of energy drinks among college female students in Jazan University such as Bison, Power Horse and Red Bull which may effect on blood chemistry, and determine correlation of consumption with study pattern, urination, health problems, heart pulses, nausea, abdominal pain, diabetes, menstrual period problems and headache. We made questionnaire that assessed consumption patterns of energy drinks among these students and withdraw blood samples for analysis. The consumers were divided into two groups regular and irregular consumers. This study showed that energy drinks are diuretic. There is a significant relation between energy drink consumption and health problems, and it also proved the strong effect of energy drinks stoppage for regular consumers. There is no effect of energy drink on blood chemistry and on Follicle stimulating Hormone.

Khat chewing is a social habit which has stimulatory action due to its cathinone content, but its adverse effects on health are a source of growing concern. The aim of our study is to evaluate the prevalence and health adverse effects of khat chewing among students in Jazan region in the Kingdom of Saudi Arabia (KSA). The study sample included 195 students from Applied Medical Science College, who were randomly selected and were asked through questionnaire and with a signed consent. About 5 ml of venous blood was collected in plain vacutainer tubes from 38 khat chewers and 20 non khat chewers as normal control. Serum was used to determine alanine aminotransferase (ALT), alkaline phosphatase (ALP), total and direct bilirubin, total protein, urea, creatinine, uric acid, and albumin. The sample consisted of 134 males and 61 females, with age range 19-27 years, and their mean age was 21.1 year. 40% of students were from urban area, and 81.5% of them from Jazan region. Out of 195 students, only 38 (19.5%) were found to chew khat. Biochemical results revealed highly significant differences among chewers in ALT, ALP, uric acid, and urea (p<0.005) compared to nonchewers group. There is also a significant difference in the total protein level (<0.05), while no significant differences were noticed in other biochemical traits analyzed. We concluded that the prevalence of khat chewing among students is fairly high (19.5 %), and that adverse effects of khat chewing on health are very clear, so all efforts should be contributed to solve this problem by increasing awareness of all members of the society to khat chewing risk.