Tiempo de latencia para semen colectado de Colossoma macropomum "Gamitana" en solución sacarosa[Latency time for semen collected form Colossoma macropomun "Gamitana" in sucrose solution] ResumenEl objetivo fue estimar el tiempo de latencia (almacenamiento), para el semen de Colossoma macropomum, "gamitana" en solución de 400 mM de Sacarosa. Se consideró aceptable los niveles de motilidad superiores al 40%, lo cual garantiza eficientes tasas de fertilización. Para el desarrollo del experimento se colectó 2 lotes de semen inmótiles de gamitana (inducidos con Conceptal ® ), los cuales posteriormente fueron activados con agua destilada. El primer lote estuvo constituido por semen en sacarosa 400 mM, puro, a temperatura ambiente y refrigerado (4°C). La motilidad fue evaluada, cada hora, hasta la 7 ma hora post colecta. El segundo lote con un semen en sacarosa 400mM a temperatura refrigerada y evaluada cada 12 horas. Los resultados del primer lote de semen demuestran que a partir de la 7 ma hora hacia delante los índices de motilidad caen significativamente por debajo del 40%. Los resultados del segundo lote demuestran la viabilidad de utilizar solución de sacarosa, como medio de conservación, para mantener semen refrigerado por 2 días y activarlos con agua destilada. El proceso de extraer y colocar repetidas veces la misma muestra en refrigeración, limita el tiempo de viabilidad de semen con sacarosa en 8 horas aproximadamente. La utilización de sacarosa como medio para almacenar semen inmotil viable de gamitana, ayuda a conservar los espermatozoides por tiempos relativamente cortos. AbstractThe aim was to estimate the latency time (storage) for semen of Colossoma macropomum, "gamitana" in solution of 400 mM sucrose. Levels of motility higher than 40% were considered as acceptable, because guaranty efficient fertility rates to fingerlings production. In order to develop this experiment, there were collected 2 lots of sperm inmótiles of gamitana (induced with Conceptal ® ) which were subsequently activated using distilled water. The first batch consisted of semen in sucrose 400 mM, pure, at environment temperature and refrigerated (4 °C). The motility was evaluated, each hour until the 7th hour post collection. The second batch included one treatment with 4 replicates of semen in sucrose 400 mM at refrigerated temperature; this batch was evaluated each 12 hours. The results in the first batch of semen shown that after the 7th hour and forward, the motility levels in significant way dropped down below to 40%. For the second batch, the results demonstrate the feasibility of using sucrose solution as a means of preservation, to keep sperm refrigerated for 2 days, and activated with distilled water. Repeated process of extracting and loading from the refrigeration, the same samples, reduce in about 8 hours the viability period in semen with sucrose. We suggest that the use of sucrose as a substrate to store inmotil sperm of gamitana helps preserving sperm for relatively short periods of time.
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