150 Samples have been collected, through the period from January/2016 to June/2016. This for isolation and identification of pathogenic gram negative bacteria. The samples were involve 50 burns samples for isolate Pseudomonas aeruginosa, 50 stool samples for isolate Escherichia coli and 50 urine samples for isolate Enterobacter cloacae and Proteus mirabilis. The isolates were identified by ten biochemical tests as well as the sensitivity and resistant were tested by thirteen type of antibiotics. These antibiotics explained different mode of action in their activity, were resistant, moderate and sensitive, some of them have significant differences when comparison together and some have no. The Trimethoprim, Amikacin, Aztreonam and Novobiocin have high activity against Ps. aeruginosa, E. coli, E. cloacae and P. mirabilis respectively, as well as the Aztreonam was more active when comparison the activity of these antibiotics together.

Background: An ear infection, can be classified into otitis externs and otitis media, this affects all age groups especially infants and young. This infection associate with pathogenic microorganism type, frequent antibiotic uses, health care and age. The most common pathogenic bacteria of the ear infection are Pseudomonas spp. Antibiotic resistance represents a serious threat to the health of humans. Methods: 48 ear swabs were collected through the use of wooden sticks in a sterile container for the identification of Pseudomonas, Serratiaand Klebsiella species by VITEK 2 system and biochemical tests. The antibiotic susceptibility against these bacterial species was detected through the method of standard disk diffusion on the Moller Hinton agar. Results: Among ear swabs, the positive growth percentage of the pathogenic gram-negative bacteria was 29.166%, and the percentage of Pseudomonas spp. was 57.142%. Males were found to be more susceptible than females with an infection percentage of 57.142%. Conclusion: The antibiotic susceptibility patterns show the azithromycin, gentamycin, piperacillin, ceftriaxone, cefepime, imipenem, ceftriazon, and gentamycin have activity against Pseudomonas spp.

In order to study the complement components (C3,C4) and c-reactive protein (CRP), serum samples were obtained from patients infected with hepatitis type C, admitted to Marjan Hospital in Province of Babylon. The results indicated significant decrease in concentrations of both complement components of patients compared with apparently healthy persons. The components (C3) concentrations were 134.875, 136.916 and 132.833 mg/dL for all patients, males and females patients respectively, while the components (C4) concentration were 30.251, 34.501 and 26.001 mg/dL for all patients, males and females patients respectively. The results also showed that the percentages of positive c-reactive protein (CRP) were 33%, 50% and 17% for all patients, males and females patients respectively.

One hundred and sixty-nine samples of urine have been collected through the period from December/2016 to May/2017. Were for isolation and identification of Proteus mirabilis. Isolated from urine in patients with UTI. Identification was done by grow-  ing on different media and biochemical tests as well as the antibiotics susceptibility were studied by using twenty types of antibiotics at acidic pH, neutral pH and alkaline or basic pH by disc diffusion method. These antibiotics were involved Ciprofloxacin, Amikcin, Meromenem, Imipenem, Ampicillin, Rifampin, Gentamicin, Trimethoprim, Tetracycline, Amoxicillin, Sulfamethoxazole, Carbnicillin, Rifaximin, Penicillin-G, Ox- olinic acid, Bacitracin, Clindamycin, Erthromycin, Novamicin and Aztreomycin. The Ciprofloxacin and Aztreomycin have high activity at both acidic and neutral pH, while the Amikcin, Meromenem, Imipenem and Ciprofloxacin have high activity at basicity pH against these bacteria.

Pseudomonas aeruginosa is an opportunistic gram-negative bacterium, which associates with a wide range of diseases. This bacterial type is found commonly in the environment, such as soils and water. Also, it represents the main cause of hospital-acquired infections, which was associated with high rates of morbidity and mortality among aging and/or immunocompromised patients. Ps. aeruginosa contains virulence factors that made them able to transmit and also gave them the ability to resist many types of antibiotics. Therefore, it represents a major challenge for health workers. One of the main characterizations of Pseudomonas aeruginosa is antibiotic resistance; this is associated with many types of genes that are encoded for different antibiotic resistance mechanisms. The efflux pump is a very important mechanism of this bacteria. Ps. aeruginosa can cause a wide range of diseases, such as pneumonia, urinary tract infections, ear infections, folliculitis, puncture wounds, cystic fibrosis, burn infections, endocarditis, endophthalmitis, meningitis, and many others.