Background:Toxoplasmosis is a parasitic disease which may cause some laboratory symptoms in infected individuals. One of the main ways to transmit this organism is placenta to the fetus pathway. If this transmission occurs in the 3rd month of pregnancy, the abortion, central nerve system and ocular disorder will happen. Because of this issue, the precise technique for the detection of Toxoplasma antibodies such as immunoglobulin G (IgG) and immunoglobulin M IgM are important, as they contain ELISA and ELISA avidity.Materials and Methods:In this survey, the main samples are serum and amniotic fluid that were collected from 48 pregnant women infected with Toxoplasma gondii in Shariaty hospital. This survey is attempted to design ELISA avidity kit in Tarbiat Modates University.Results:The results from this survey show that, in these total pregnant women the infection by T. gondii has occurred and many of them are infected currently.Conclusions:In the simple ELISA technique, the only antibody that can be detected precisely is IgM; however, using this technique the IgG antibody can also be detected. In this new technique or ELISA avidity, in addition to detection of IgG antibody against T. gondii, the month of transmission of Toxoplasma is also interpreted.

Background: Toxoplasma parasite is from Toxoplasmatidea family that initially was seen in ctinodactylus gondii rodent. Toxoplasma parasites that extracted from different rodents are same in immunologic and morphologic characteristics but have differences in pathogenicity and genotypes in mice. The rodents and cattle are most reservoir hosts in environment that by attention of human environment vicinity to cattle and rodent's environment causes Toxoplasma dispersion in that area. The aim of this study was Molecular detection of Toxoplasma gondii in rodents and cattle of Golestan province, northeast of Iran.

Background and aims:Toxoplasmosis is a very common disease in the world. Two types of acquired toxoplasmosis have been detected. In the chronic toxoplasmosis, the abnormality of tissue function is little but, in acute toxoplasmosis, function of RES system becomes interrupted. The assessment of toxoplasma antibody with tissue enzymes in this stage is very important. Furthermore, serum ferritin in some conditions became high in acute phase of infectious disease. Methods: This study was based on comparative abundance study. Materials consisted of 980 serum and amniotic fluid samples collected from human blood with high level of IgG antibody against Toxoplasma gondii in Rajaie center, Tehran, Iran. The standard test was ELISA assay to detect these antibodies and the main test was measurement of liver transaminases (SGOT, SGPT) bilirubin and ferritin to evaluate acute toxoplasmosis. Data analysis was done by SPSS version 18.0. This study was done during March to June, 2017. Results: Results showed that in some patients with high level of IgG AVIDITY antibody against T. gondii the level of liver transaminases, serum bilirubin and ferritin became increased. For example in 120 patients with acute toxoplasmosis the mean serum levels of SGOT were 108 IU/L and in 80 patients the mean serum bilirubin were about 5 mg/dL. Conclusion: In acute congenital toxoplasmosis, the evaluation of IgG AVIDITY was first step and then the measurement of biochemical factors such as serum transaminases, serum Bilirubin and serum Ferritin were important.