Demand for duck eggs and meat recently increased along with the increasing interest of the community to consume duck eggs and meat. Due to that reason researcher try to find the best way to enhance the reproductive ability of livestock., which is in this research by laser puncture shot. Eighteen ducks were allocated into three groups, the control group, the first treatment group with laserpuncture shoot dose of 0.2 Joule ( J), and the second treatment group with laser puncture shoot dose of 0.4 J. Data were analyzed with ANOVA and further tested by Duncan multiple range test. Research result showed that laser puncture shoot with a dose of 0.2 J and 0.4 J decreased the number of Sertoli and Leydig cells.

The aim of this research was to explore the influence of Mozart KV 448 classical music therapy to the neuroglia cells of mice's hippocampus that were exposed to stress during prenatal. This research were employing twenty female mices and twenty male mices. Female mices were estrus synchronized with PMSG and hCG then monomating to the males. Pregnant females were then divided into four groups (P0, P1, P2, P3). P0 was as control, P1 was treated by one minute forced swim test, P2 was treated by one minute forced swim test followed by thirty minutes classical music Mozart KV 448 and P3 was treated by one minute forced swim test followed by sixty minutes classical music Mozart KV 448. This research was carried out for twenty-one days during gestation period. The neuroglia density result was analyzed using ANOVA and Duncan test. The neuroglia activity result was analyzed using Kruskal wallis test and Z test. The histology reading showed degradation of density and activity of hippocampus neuroglia.Keywords: prenatal stress, classical music, Mozart KV 448, neuroglia, hippocampus AbstrakTujuan dari penelitian ini adalah untuk mengetahui pengaruh terapi musik klasik Mozart KV 448 pada densitas dan aktivitas dari sel neuroglia hipokampus anak mencit yang terpapar stres selama prenatal. Penelitian ini menggunakan 20 mencit betina dan 20 mencit jantan. Mencit betina kemudian di sinkronisasi estrus dengan PMSG dan hCG setelah itu dilakukan mono mating. Mencit betina yang positif bunting di bagi menjadi empat kelompok (P0, P1, P2, P3). P0 sebagai kontrol tidak diberikan perlakuan apapun, P1 diberikan terapi forced swim test selama satu menit, P2 diberikan terapi forced swim test selama satu menit diikuti musik klasik Mozart KV 448 selama 30 menit dan P3 diberikan terapi forced swim test selama satu menit diikuti musik klasik Mozart KV 448 selama 60 menit. Penelitian ini dilakukan selama 21 hari periode kebuntingan. Analisis data pada densitas neuroglia menggunakan ANOVA dan uji Duncan selanjutnya untuk aktivitas neuroglia data yang diperoleh berupa skor nilai tingkat perubahan aktivitas antar neuroglia sehingga menggunakan uji Kruskal wallis dan uji Z. Hasil gambaran histologi dari penelitian ini menunjukkan penurunan terhadap densitas dan aktivitas neuroglia hipokampus.