BACKGROUND: The COVID-19 pandemic has a major impact on health workers’ mental health. Health workers in Indonesia have received COVID-19 vaccines to get protection against COVID-19 infection and reduce anxiety while on duty. AIM: This study aims to determine anxiety disorder in health workers at public health centers who have received COVID-19 vaccines. METHODS: A cross-sectional study was conducted at six public health centers in Tegal district, Indonesia. Data were collected using questionnaire that given to health workers who had vaccinated COVID-19. Anxiety disorder was determined with generalized anxiety disorder-7 (GAD-7) questionnaire. RESULTS: There were 137 samples who participated in this study, consisted of 83.2% of women and 16.8% of men. The majority of the samples were 30−39 years old (41.6%), married (93.4%), and midwife (44.5%). There were 6.6% of samples had mild anxiety and others were normal (93.4%). CONCLUSION: COVID-19 vaccination program for health workers can reduce anxiety when providing health services. However, assistance is still needed for health workers who have mild anxiety disorder to prevent prolonged symptoms and more severe mental health condition.