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Interleukin-8 (IL-8) is angiogeneic chemokine that plays a potential role in both development and progression of many human malignancies including nasopharyngeal carcinoma (NPC). Epstein- Barr virus (EBV) is recognized to be an important etiologic agent of NPC as the viral gene products are frequently detected in NPC tissue along with the elevation of antibody titre to the viral protein (VCA-p18+ EBNA1) of IgA in the majority of patients. Elevated plasma of Viral Load is regarded as an important marker for the presence of the disease and for the monitoring of disease progression. However, other serum/plasma parameters such as the level of certain interleukins (IL-8 and IL-10) has also been implicated in NPC progression. The study aimed to investigate the correlations between plasma Viral Load and the level of interleukin (IL-8) and Interleukin (IL-10) in relating these parameters to the stages of NPC. In addition of Viral Load (VCA-p18+EBNA1) IgA, Interleukin-8 and Interleukin-10 before and after therapy will be investigated to seek the possible marker for disease progression. A total of 39 NPC patients and 29 healthy control individuals enrolled in this study. Plasma Viral Load was quantified using real-time quantitative PCR. The Level of plasma interleukins both IL-8 and IL-10 were analyzed using ELISA methods. Results indicated there was a significant decrease in viral load was detected in plasma of NPC patients following therapy. Plasma of viral load was shown to be a good prognosticator for disease progression. There were positive correlation between plasma of viral load and IL-8. These non invasive parameters expressed in blood, could be substitutes of viral load using brushing method, which is invasive. In conclusion that: Viral Load, (VCA-p18+EBNA1) IgA and IL-8 levels are promising markers for the presence of NPC and progression of the disease.

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Nasopharyngeal cancer is the fifth most severe malignant disease in the head and neck in the human body. The main treatment is chemoradio therapy. The protein gene 53 (p53) is a tumor suppressor gene. YinYang1 (YY1) is a transcription factor having an important role in cell cycle control. YY1 can function as an activator, suppressor or initiator of the gene transcription process. This research aims to see the relationship between p53 mRNA gene expression and YY1 mRNA gene expression on the NPC TNM Stage. Materials andmethods cross-sectional research at 20 WHO type 3 NPC in which 3 samples after chemoradiotherapy, 17 non-chemoradio therapy samples consisted of 8 stage two samples, 7 stage three samples and 2 stage four samples. With RT-PCR, YY1 mRNA gene expression and p53 mRNA gene expression were measured, then the T-test was independent of the average chemoradio therapy group. It was concluded, at a higher NPC stage, the level of YY1 mRNA gene expression was relatively higher while p53 expression was lower. Post-chemoradio therapy levels of p53 mRNA gene expression were higher and YY1 expression was lower than in the non-chemoradio therapy group.

Latar belakang: Karsinoma nasofaring (KNF) adalah keganasan epitelial yang banyak dijumpai pada populasi Cina dan Asia Tenggara termasuk Indonesia. Virus Epstein Barr (EBV) terbukti berasosiasi kuat dengan karsinoma nasofaring. Beberapa protein virus seperti EBER, LMP1, LMP2 ditemukan pada jaringan dan darah penderita kanker nasofaring. Selain itu terjadi peningkatan kadar antibodi terhadap protein virus IgA (VCA-p18+EBNA-1) pada mayoritas pasien. Peningkatan plasma EBV DNA merupakan marker yang penting adanya penyakit dan untuk monitoring progresifitas penyakit. Parameter lain serum/ plasma yaitu kadar Interleukin-8 (IL-8) dan interleukin-10 (IL-10) juga terlibat didalam progresifitas karsinoma nasofaring. Tujuan: penelitian ini untuk melihat hubungan kadar IL-8 dan IL-10 dengan stadium dari karsinoma nasofaring. Metode: Penelitian ini bersifat potong lintang (cross sectional study) pendekatan survei eksploratif dengan 39 pasien karsinoma nasofaring dan kontrol 29 orang sehat dalam penelitian ini. Nilai kadar plasma IL-8 dan IL-10 diperiksa dengan ELISA. Hasil: menunjukkan terdapat korelasi positif antara IL-8 dan IL-10 terhadap progresifitas KNF. Kesimpulan: IL-8 berhubungan dengan progresivitas karsinoma nasofaring. Rasio IL-8 dan IL-10 dapat digunakan menilai prognosis KNF. Bila hasil rasio IL-8:IL-10>1 menunjukkan tendensi buruk, oleh karena itu mungkin dapat diusulkan sebagai faktor prediktor karsinoma nasofaring. Kata kunci: Karsinoma nasofaring, IL-8, IL-10.0 ABSTRACTBackground: Nasopharyngeal cancer (NPC) is an epithelial malignancy, prevalent in Chinese populations and Southeast Asia including Indonesia. Epstein Barr Virus (EBV) has been assosiation with nasopharyngeal cancer. Viral gene products namely EBER. LMP1, LMP2 and EBNA’S have been found in nasopharyngeal cancer tissues. In addition increase of IgA (VCA-p18+EBNA-1) in the majority of patients. most often found in nasopharyngeal cancer tissues with an increase in IgA antibody titer of viral proteins (VCA-p18+EBNA -1) in the majority of patients. The increase of plasma of EBV DNA load is an important marker of disease and for monitoring its progression. Other parameters of serum/plasma is level of Interleukin-8 (IL-8) and interleukin-10 (IL-10) which also involved in nasopharyngealcancer progression. Purpose: to find out the relationship level of interleukin-8 (IL-8) and interleukin-10 (IL-10) in relation with the stadium of nasopharyngeal cancer. Method: A cross sectional study with exploratif survey was conducted 39 patients of NPC, controls 29 healthy subjects included in this study. The value of plasma levels of IL-8 and IL-10 examined by ELISA. Result: There was a positive correlation between IL-8 and IL-10 against the progression of NPC. Conclusion: IL-8 and is a marker of NPC and the progression of the disease. The ratio of IL-8 and IL-10 can be used to assess prognosis of NPC. Ratio of IL-8:IL-10 > 1 indicates a poor prognosis. Keywords: Nasopharyngeal, cancer, IL-8, IL-10