The cultivation of shiitake mushroom Lentinula edodes is very important all over the world, due to its nutritional properties. The available studies on the chemical characterization of local shiitake strains that grow in the Iraqi environment and cultivated in the laboratory are not available so far, therefore, the aim of the current work is to quantitative and qualitative determination of amino acids and fatty acids in the strain L. edodes ( OM432157 ) grown in vitro and wild using amino acid analyzer and gas chromatography ( GC ). The results showed that the percentage of total EAA in the fungal strain L. edodes ( OM432157 ) amounted to 37.14% and in the wild strain ( 41.9% ). The predominant essential amino acid in the strain L. edodes was valine. While the proportion of TNEAA in the cultivated strain L. edodes (OM432157) was 56.27% and the wild strain 51.05%, cysteine acid was the predominant component in the cultivated strain, in contrast, glutamic acid was the dominant acid in the wild strain Regarding the fatty acid profile, the unsaturated fatty acids were dominant over the saturated fatty acids in both types of mushrooms. It was observed that PUFA was higher than MUFA in the cultivated strain, and Linolenic acid ( C18:3 ) was the dominant acid and the form 47.6 % of the total fatty acids in contrast to the wild strain which recorded a high percentage of MUFA 83% compared to PUFA12.6%, and palmitoleic acid ( C16: 1) is dominant and constituted 81.9% of the total fatty acids. Our findings contributes to the determination of amino acids and fatty acids in the local strain L. edodes ( OM432157).

Shiitake mushroom Lentinula edodes have been a part of the human diet since ancient times. Mushrooms have gained a lot of attention lately due to their prospects in medicine and nutrient production. Due to the lack of research studies on this fungus globally, particularly in Iraq, this study is considered the first in which agricultural waste is used to produce local food mushrooms. The local strain L. edodes (OM432157), which was discovered for the first time in the Iraqi environment, was cultivated in the laboratory using nine treatments based on sawdust as the main substrate. It was observed that the highest growth rate of the fungus strain under study was in T8 (1.8 cm) and the lowest growth rate in T5 was 1.1 (cm). Fungal tissue growth in treatments (T4, T5) required (29 days), while in treatment T8 it required (26 days), and in other remaining treatments it accomplished in 25 days. The time required for pin heads to form in T5 was obviously faster (17 days), followed by T8 (18 days) and T9 (19 days). In addition, that both treatments (T5, T9) achieved a high yield of mushroom, as T5 was 250 gm, and treatment T8 reached 200 gm. Our findings contributes to product the local strain L. edodes (OM432157) wild in the laboratory by using different substrates.