Khamer (Gmelina arborea) is a fast-growing multipurpose tree belongs to the family Lamiaceae (Verbinaceae). It has multiple uses ranging from particle board, plywood, core stock, furniture, carriages, sports, musical instruments to sawn. It is mainly propagated through seeds but the problem in seed germination is its hard seed coat which limits seed germination. So the present study was carried out at experimental area of Agroforestry plot of Tropical Forest Research Institute, Jabalpur in the year 2021 with an aim to find best pre-sowing treatment for enhancement of germination in G. arborea. Seeds were treated with different treatment includes growth regulators (GA3 100 and 200 ppm), acid scarification (H2SO4), cold water and untreated seeds as control. Among all the treatments seeds treated with GA3 (200 ppm) recorded the higher germination percentage (98.88%) followed by H2SO4 (93.33%) as compared to untreated seeds in control.