: In late 2019, a novel coronavirus named COVID-19 led to a large outbreak in China and many other countries. A few cases of pneumonia in newborns and infants with COVID-19 infection have been reported. The neonates and infants described as the cases of COVID-19 had been asymptomatic or have had mild symptoms. The target of the current report is a 35-day-old male infant with respiratory distress and cyanosis. The chest x-ray CT images revealed manifestation of lung infection. The upper respiratory sampling was done by pharyngeal swab and the results confirmed the COVID-19 infection. Considering the positive test results and the severity of the respiratory distress, a complex medication treatment was administered. As a result, the symptoms alleviated, and the patient was discharged after complete remission on the 11th day. Although rare cases of COVID-19 infection in infants have been reported, the transmission of disease from affected persons to infants can happen. Therefore, further studies for early diagnosis and management of the COVID-19 in newborns and infants are necessary.

This study investigated how the time interval between the last EMS (netting) and the acute confinement stress (AC stress) at the end of the experiment can influence growth, haematology, blood biochemistry, immunological response, antioxidant system, liver enzymes, and stress response of oscar (Astronotus ocellatus; 5.7 ± 0.8 g). Nine experimental treatments were tested, as follows: Control, Stress28 (EMS in weeks two and eight), Stress27 (EMS in weeks two and seven), Stress26 (EMS in weeks two and six), Stress25 (EMS in weeks two and five), Stress24 (EMS in week two and four), Stress23 (EMS in week two and three), Stress78 (EMS in week seven and eight), and Stress67 (EMS in week six and seven). After the nine-week experimental period, while it was not significant, fish exposed to Stress78 (26.78 g) and Stress67 (30.05 g) had the lowest growth rates. After AC stress, fish exposed to Stress78 (63.33%) and Control (60.00%) showed the lowest survival rate. The Stress78 fish displayed low resilience, illustrated by values of blood performance, LDL, total protein, lysozyme, ACH50, immunoglobin, complement component 4, complement component 3, cortisol, superoxide dismutase, catalase, and alanine aminotransferase. In conclusion, gathering consecutive stress and not enough recovery time in the Stress78 group negatively affected stress responsiveness and the health of oscar.

Measles is an infectious febrile sickness. Despite the existence of effective vaccination, eradicating measles remains a global priority. It is required to set up a sensitive and specific test to identify measles-specific antibodies in order to estimate the level of community protection against measles. Although the plaque reduction neutralization test (PRNT), the gold standard test, is technically challenging, it is not suitable for routine use. The enzyme-linked immunosorbent assay (ELISA) was also unable to detect weak antibody responses in people vaccinated against measles. As a result, the FRNT (focus reduction neutralization test) was established and validated. In eight assay runs, the samples were analyzed in triplicate by PRNT, FRNT, and ELISA. In a total of 50 samples analyzed, 38/50 samples were positive by standard PRNT, 37/50 samples by FRNT, and 19/50 samples were positive by ELISA. The results showed that ELISA was not sensitive enough to identify low levels of anti-measles antibodies, and that ELISA and both neutralization assays had a weak agreement. The two neutralization assays, on the other hand, had a perfect correlation. Finally, the PRNT and FRNT have similar sensitivity. For characterizing the immunological response to measles and vaccination efficacy trials, FRNT seems to be a good alternative to PRNT. Therefore, it will be very important to validate the negative and equivocal ELISA results with this standard test in the final stages of eradication.