Island species are susceptible to extinction through disturbances such as habitat transformation. Due to the small size and isolation of islands, species have limited options for refuges and recolonization, making their rehabilitation a conservation priority. Robben Island is a continental island, isolated from the mainland ca. 15 000 years ago, and has been degraded by humans and alien species for nearly 400 years. Mainland areas with similar vegetation should be good reference sites for the biological restoration of the island due to historical connectedness. However, very little information exists as to which species were lost. Here we aim to identify the best mainland sites to use as reference sites for Robben Island based on remaining arthropod diversity on the island. Sites found to be most similar in terms of arthropod diversity to Robben Island were sites north of Robben Island (Elandsbaai and Dwarskersbos) rather than the geographically closest locations. These sites therefore represent ideal reference sites for biological restoration of the island. We do not suggest the reintroduction of species from these localities, but rather Robben Island should be restored to match their vegetation height and cover.