The purpose of the work is to develop methodological foundations for optimizing the concentration of hydroaeroions from the variability of the physical factors of the working room airspace with ultrasonic ionization. Methodology. The measurements were carried out according to the developed methodology with the “Sapphire 3K” air ion counter and with the use of a TM-4001 hot-wire anemometer. To analyze the data of experimental studies, the method of mathematical planning of the experiment was used. The scheme of rotatable central compositional planning (RCSC) was used, which is based on regression analysis, including the method of least squares and statistical data processing. For all measurement results, the adequacy of the regression models was checked using the built-in statistical functions of Statgraphiks. Results. Experimental studies have shown that the use of an ultrasonic generator of air ions significantly increases the concentration of negative ions (2400 cm-3) and that is the increase in the quality of the internal working space, which is responsible for the production of ozone and nitrogen oxide. Mathematical processing of the obtained data made it possible to establish the general effect of the air flow in the room (v) and the distance to the UAG (s) on the concentration of positive and negative aerons. The influence of varying each of the studied factors on the value of the concentration of air ions was quantitatively evaluated. Scientific novelty. The synthesized regression models of hydroaerion concentrations describe the relationship between the air speed in the room and the distance to the ultrasonic generator of air ions. The adequacy of the models was checked with the coefficient of determination R2 = 86%, which indicates a high degree of connection between the coefficients of the system. Practical significance. The result obtained can be used in the design and development of a control system for an ultrasonic generator of air ions in order to create the most comfortable high-quality ionic composition of air in the working area.