Comprehensive two-dimensional gas chromatography (GC×GC) based on flow-modulation (FM) is gaining increasing attention as an alternative to thermal modulation (TM), the recognized GC×GC benchmark, thanks to its lower operational cost and rugged performance. An accessible, rational procedure to perform method translation between the two platforms would be highly valuable to facilitate compatibility and consequently extend the flexibility and applicability of GC×GC. To enable an effective transfer, the methodology needs to ensure preservation of the elution pattern, separation power, and sensitivity. Here, a loop-type thermal modulation system with dual detection (TM-GC×GC-MSD/FID) used for the targeted analysis of allergens in fragrances is selected as reference method. Initially, six different columns configurations are systematically evaluated for the flow-modulated counterpart. The set up providing the most consistent chromatographic separation (20 m x 0.18 mm d c x 0.18 μm d f + 1.8 m x 0.18 mm d c x 0.18 μm d f) is further evaluated to assess its overall performance in terms of sensitivity, linearity, accuracy, and pattern reliability. The experimental results convincingly show that the method translation procedure is effective and allows successful transfer of the target template metadata. Additionally, the FM-GC×GC-MSD/FID system is suitable for challenging applications such as the quantitative profiling of complex fragrance materials.

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