BackgroundThe aim of our research work was to quantify total flavonoid contents in the leaves of 13 plant species family Asteraceae, 8 representatives of family Lamiaceae and 9 plant species belonging to family Rosaceae, using the multiplex fluorimetric sensor. Fluorescence was measured using optical fluorescence apparatus Multiplex(R) 3 (Force-A, France) for non-destructive flavonoids estimation. The content of total flavonoids was estimated by FLAV index (expressed in relative units), that is deduced from flavonoids UV absorbing properties.ResultsAmong observed plant species, the highest amount of total flavonoids has been found in leaves of Helianthus multiflorus (1.65 RU) and Echinops ritro (1.27 RU), Rudbeckia fulgida (1.13 RU) belonging to the family Asteraceae. Lowest flavonoid content has been observed in the leaves of marigold (Calendula officinalis) (0.14 RU) also belonging to family Asteraceae. The highest content of flavonoids among experimental plants of family Rosaceae has been estimated in the leaves of Rosa canina (1.18 RU) and among plant species of family Lamiaceae in the leaves of Coleus blumei (0.90 RU).ConclusionsThis research work was done as pre-screening of flavonoids content in the leaves of plant species belonging to family Asteraceae, Lamiaceae and Rosaceae. Results indicated that statistically significant differences (P > 0.05) in flavonoids content were observed not only between families, but also among individual plant species within one family.
Leaf area duration of oilseed rape (Brassica napus subsp. napus) varieties and hybrids and its relationship to selected growth and productivity parametersSumárna listová pokryvnosť líniových odrôd a hybridov kapusty repkovej pravej (Brassica napus subsp. napus) a jej vzťah k vybraným rastovo-produkčným ukazovateľom shown as a presumption for high production potential of winter oilseed rape genotypes.
The collection of 10 parental lines of tetraploid wheat genotypes with various origin was cultivated in growth chamber. Leaf and growth traits such as assimilation pigments content, dry mass and leaf area of plants were measured. The genotype differences were recognized by chlorophyll a fluorescence fast kinetics method applied on penultimate young wheat leaves. Consequently, heat stress susceptibility based on the exposure of whole plants in pots to air temperature of 42 o C for 6 hours was measured, too. Rapid chlorophyll a fluorescence kinetics method gave us better knowledge about differences among tetraploid wheat genotypes collection comparing to classic assimilation pigments analyse. Genotypes with higher content of pigments did not always exhibit good resistance against heat stress. Using the chlorophyll fluorescence parameters, the rating of genotypes based on photosynthetic performance as well as photosystem II (PSII) thermostability was done. We identified the genotypes TRG7 (Triticum turgidum subsp. turgidum, PI 384230, Ethiopia) and RONCAL (Triticum turgidum subsp. dicoccon, TRI 17700, Spain) as the perspective donors of genes for better thermostability of photosynthetic apparatus in changed climate conditions and material into wheat efficiency breeding programs.Keywords: chlorophyll a fluorescence, heat stress, JIP-test, photosynthetic performance, photosynthetic pigments, photosynthetic traits, tetraploid wheat genotypes Abstract in Slovak languageKolekcia 10 rodičovských línií tetraploidných pšeníc rôzneho pôvodu bola pestovaná v rastovej komore. Merané boli listové a rastové charakteristiky, obsah asimilačných pigmentov, suchá hmota a listová plocha rastlín. Zisťované boli aj genotypové .s -1 na úrovni listov. Po 6 týždňoch bol zisťovaný obsah asimilačných pigmentov, suchá hmota, listová plocha rastlín a merané vybrané parametre fotosyntézy aplikáciou fluorescenčnej techniky na nestresované rastliny. Následne boli rastliny podrobené teplote 42 o C v rastovej komore na svetle počas 6 hodín, závlahou a prechodným zvýšením vlhkosti vzduchu bolo zabránené dehydratácii listov. Následne boli mladé, plne vyvinuté predposledné listy (po predchádzajúcej 30 minútovej adaptácii na tmu pomocou klíps zadržiavajúcich svetlo) merané metódou rýchlej kinetiky fluorescencie chlorofylu a na. Indukčné krivky fluorescencie chlorofylu a boli získané pomocou prenosného fluorimetra Handy PEA (Plant Efficiency Analyser, Hansatech Instruments Ltd., Kings Lynn, UK). Takto získané rýchle fluorescenčné prechody (tranzity) boli analyzované tzv. JIP testom. Merané a počítané parametre JIP-testu sú zobrazené v tab. 1. Obsah chlorofylu je zvyčajne v korelácii s fotosyntetickou výkonnosťou a úrodovým potenciálom. Produkcia (tvorba) vysokej biomasy závisí od veľkosti listovej plochy a jej štruktúry, zahŕňajúc obsah chlorofylov, karotenoidov a ich funkčných prejavov. Podľa nameraných hodnôt koncentrácie pigmentov, suchej hmoty a listovej plochy rastlín v tab. 2 môžeme povedať, že genotypy ISP6 a TRG7 dosiahli v týchto para...
HUNKOVÁ, E., WINKLER, J., DEMJANOVÁ, E.: The weed seed bank assessment in two soil depths under various mineral fertilising. Acta univ. agric. et silvic. Mendel. Brun., 2011, LIX, No. 5, pp. 105-112 The fi eld trial at the experimental station of Slovak Agricultural University in Nitra -Kolíňany (Slovak Republic, maize growing region, Haplic Luvisol and Stagni-Haplic Luvisol) in 1997 year was established. Experiments were based on 14 ha area (424 x 432.2 m) by long strips method. The impact of diff erent mineral fertilisers on six model crops was observed: winter wheat, spring barley, sunfl ower, winter oilseed rape, maize and sugar beet. weeds, mineral fertilising, weed seed bank Weedy plants overcome spatial and time distances in the fruits and seeds form; thereby, they determinate the state and weed infestation changes on arable land. The increase of weed population growth is conditioned by weed seeds and fruits input inside of arable land. It is accomplished by weed seeds dropping on concrete site, by seeds from organic fertilisers, by seeds for sowing and by uncontrollable inputs (wind, undomesticated animals). The reduction of weed infestation is given by decrease of dropping weed seeds on concrete site, by weed suppression and control, by increase of soil's self-cleaning ability and by weed seeds minimizing in materials that transport seeds (organic fertilisers, seeds for sowing). Weed infestation decreases when the reduction of seeds exceeds their entry into the soil, only. Viable seeds in the soil's reserve (i.e. weed seed bank) are the fi rst assumption of actual weed infestation (Dvořák, Smutný, 2003).Weed fruits and seeds are a viable reservoir in the upper part of the soil profi le, which determinates the composition of weed fl ora in the concrete region (Caetano et al., 2001). Management of agricultural systems has immediate and long-lasting eff ects for elements of weed coverage, population and diversity. Development of programs for integrated weed control requires a clear understanding of factors and mechanisms determinative the weed community dynamics in agroecosystems (Menalled et al., 2001).The goal of this work was to discover the impact of mineral fertilisation, years of research, and the depth of weed seed off takes on weed seed soil bank in Kolíňany locality.
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