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The effect of repeated deep frying of potatoes versus repeated heating/quenching on the chemical profile of palm oil was investigated. The novelty of the work is that the frying and heating/quenching experiments were conducted under similar time-temperature profiles. The effects of the frying load (potato-to-oil ratio: 1/7 and 1/35 kg potatoes /l oil ) and of the time-temperature profile were examined. Whole palm oil and its polar fraction were analyzed using high pressure size exclusion chromatography. Both repeated frying and repeated heating/quenching generated polar and polymerization products in palm oil. Interestingly, no hydrolysis or other decomposition products were generated under any of the examined conditions. The presence of potatoes during frying in palm oil increased the concentration of polymerization products and polar compounds compared to oils without potatoes significantly. The effects of frying load on oil quality depended on frying time. No significant effect of frying load was observed up to frying times of 13 h (or 10 frying batches). However, frying oil quality was affected by frying load once frying times exceeded 24 h (or 20 batches).

The objective of this research was to perform an exposure assessment of aflatoxin M1 (AFM1) intake through the consumption of milk and yoghurt by the student population in Serbia and Greece. A food consumption survey of milk and yoghurt was performed during the first half of 2018 in the two countries with at least 500 interviewees (aged between 18 and 27 years) per country, covering their dietary habits and body weight based on one-day and seven-day recall methods. Values for the concentration of AFM1 were extracted from published research. Finally, a Monte Carlo analysis of 100,000 iterations was performed to estimate the intake of AFM1 from the consumption of the two dairy products. Results revealed that the estimated average exposure of students to AFM1 was in the range of 1.238–2.674 ng kg−1 bw day−1 for Serbia, and 0.350–0.499 ng kg−1 bw day−1 for Greece, depending on the dietary recall method employed. High estimations for hepatocellular carcinoma (HCC) cases/year/105 individuals, depending on the prevalence of Hepatitis B virus surface antigen positive individuals (HBsAg+), were 0.0036–0.0047 and 0.0007–0.0009 for Serbia and Greece, respectively. Presented Margin of Exposure (MOE) and Hazard Index (HI) values indicate increased risk from exposure to AFM1, particularly in Serbia.

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