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Side effects of fat intake and consumers’ awareness have forced the food industry to produce healthier food. In recent years, non-fat yogurt has increasingly been consumed all over the world. In this study, the effect of adding a mixture of inulin, whey protein isolate, modified starch with different levels (0.3, 0.5 and, 1%) and gelatin (0.2%) on biochemical (pH, titratable acidity (TA), and Redox potential), rheological, and sensory properties of non-fat set yogurt was investigated. The samples containing higher levels of whey protein were better treatments regarding biochemical, sensory and rheological properties. The highest syneresis was observed in control yogurts and the samples with 1% inulin, whereas no syneresis was observed in samples containing 1% starch or 1% whey protein. Overall, the yogurts containing 1% whey proteins, 0.5% modified starch, 0.3% inulin, and 0.2% gelatin showed the most desirable characteristics of non-fat yogurt.