The authors set out in this study to verify the presence of low-and high-risk DNA of human papillomavirus (HPV) in oral cancer by means of the hybrid capture Digene ® test (São Paulo-SP, Brazil) in smears from exfoliative cytology and also to compare the findings with those of conventional light microscopy (hematoxylin-eosin (HE)/Papanicolaou). Forty individuals gave their written informed consent to participate in the study and also had their clinical data analyzed. The 40 exfoliative cytology examinations performed to date produced the following results: 29 (72.5%) negative for low-and high-risk HPV-DNA; nine (22.5%) positive for low-and high-risk HPV-DNA; one (2.5%) positive for low-risk HPV-DNA; and one (2.5%) positive for high-risk HPV-DNA. There was agreement among the findings for the presence of DNA-HPV for both exfoliative cytology (smear to hybrid capture Digene ® test and the cytological smear readings made by conventional light microscopy). It was therefore concluded that the HPV virus may be a cocarcinogen of the mouth cancer as it is in the cervix cancer. Oral cancer HPV Exfoliative cytology Hybrid capture resumo Os autores buscaram verificar, neste estudo, a presença do papilomavírus humano (HPV) de baixo e de alto risco em carcinomas orais através do teste de captura híbrida Digene ® (São Paulo-SP, Brasil) em amostras colhidas pela citologia esfoliativa bucal e, ainda, avaliar comparativamente as referidas leituras com alterações celulares indicativas deste vírus obtidas com a interpretação citológica óptica convencional (hematoxilinaeosina (HE)/Papanicolaou). Quarenta indivíduos concordaram, espontaneamente, através de assinatura do termo de consentimento livre e esclarecido, em participar da pesquisa, e seus dados clínicos foram analisados. Entre as 40 amostras provenientes da citologia esfoliativa 29 (72,5%) mostraram-se negativas para presença de HPV-DNA de baixo e de alto risco; nove (22,5%) foram positivas para o HPV-DNA de baixo e de alto risco; uma (2,5%) foi positiva apenas para o HPV de baixo risco; e também uma (2,5%) foi positiva apenas para o HPV de alto risco. Houve concordância entre todos os resultados positivos para presença de HPV-DNA nas amostras citológicas submetidas ao teste de captura híbrida Digene ® e na leitura de esfregaço citológico ao microscópio óptico convencional. Desta forma, conclui-se que o vírus HPV pode comportar-se como mais um co-carcinógeno para o câncer de boca, à semelhança do carcinoma uterino.

Moreira MAR, Longato-Filho A, Taromaru E, Queiroz G, Jubé LF, Pinto SA, Schmitt FC. Investigation of human papillomavirus by hybrid capture II in cervical carcinomas including 113 adenocarcinomas and related lesions. Int J Gynecol Cancer 2006;16:586-590.Hybrid capture is an easy and highly sensitive technique for screening population due to its capacity to detect malignant and premalignant lesions of the cervix. To evaluate its sensitivity, we investigated the frequency of high-risk human papillomavirus (HPV) infection and its correlation with glandular malignant lesions, analyzing a total of 113 cases of adenocarcinomas and related lesions. High-risk HPV was investigated using a hybrid capture II (HC2) assay. Samples were collected in two different ways: either brushed directly from surgical specimens before fixation or collected from the patients. We also investigated the frequency of HPV in squamous malignant lesions, 65 squamous cell carcinomas (SCC) and 66 in situ squamous cell carcinomas (ISSCC), to compare the occurrence of HPV for these lesions. The 113 glandular lesions comprised 62 invasive adenocarcinomas (IAC), 8 in situ adenocarcinomas (ISAC), 26 IAC plus SCC, and 17 adenosquamous cells carcinomas (ASCC). The HPV-positive reactions were as follows: 51 (82.2%) in IAC, 8 (100%) in ISAC, 25 (96.1%) in IAC plus SCC, and 14 (82.3%) in ASCC. HC2-positive results in the squamous malignant lesions were as follows: 58 of 63 (89.0%) for SCC and 94 of 103 (91.2%) for ISSCC.High-risk HPV infection was quite similar for glandular and pure squamous invasive malignant lesions, 82.2% and 89.0%, respectively, indicating that high-risk HPV is also highly prevalent in glandular lesions. Although hybrid capture proved to be an excellent adjunctive technique, we do not believe its results merit replacing the Pap smear as a screening tool.KEYWORDS: adenocarcinoma, cervical intraepithelial lesion, HPV, hybrid capture II, squamous cells carcinoma.Cervical cancer is distributed throughout the world, afflicting 500,000 women annually. The most important risk factor for the development of this condition is the human papillomavirus (HPV) infection. It is believed that 80% of primary cancers begin from preexisting squamous dysplasia. With respect to glandular epithelium malignancies, adenocarcinomas account for about 20% of invasive carcinomas, which probably arise from in situ adenocarcinomas (ISAC) (1) .Interestingly, the incidence of adenocarcinoma is increasing mainly in developed countries (1,2) , and it is currently thought to be more significant in large cities due to the increased probability of HPV infections.Glandular malignant lesions, namely, ISAC, are frequently overlooked because clinical, colposcopic, and cytologic parameters are unfamiliar and bypassed by concomitant squamous alterations. Additionally, more than 50% of ISAC are mixed diseases with a squamous lesion counterpart (3) . Indeed, traditional methods have poor sensitivity to detect precursor lesions of glandular carcinomas. HPV status has a significant ...

For mild dyskaryosis, DNA-Citoliq detected 176 cases and CS 125 cases (McNemar test, P < 0.000); and for moderate+severe dyskaryosis 66 versus 32 cases respectively (McNemar test, P < 0.000).