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Aims It has been demonstrated that there are abundant stable microRNAs (miRNAs) in plasma, which is potentially disease‐specific. Adrenergic and muscarinic pathways play an important role in voiding physiology. Alterations in the levels of miRNAs are thought to influence the regulation of these pathways at the molecular level. The aim of this study was to investigate the relationship of miRNAs with overactive bladder pathogenesis and to provide a new perspective to treatment approaches. Methods This study included patients with an overactive bladder (OAB) diagnosis and a healthy control group. All patients completed a validated OAB‐V8 questionnaire. The relative expression levels of 12 miRNAs were examined in plasma by PCR. Receiver operating characteristic (ROC) curves were generated to evaluate the diagnostic qualification of miRNAs. Results The relative expression levels of let‐7b‐5p, miR‐92a‐3p, miR‐98‐5p, miR‐142‐3p, and miR‐200c‐3p were significantly upregulated and miR‐139‐5p was significantly downregulated in patients with OAB and no correlation was determined between the levels of miRNAs with OAB symptom score. Among the miRNAs, miR‐98‐5p provided the highest diagnostic accuracy alone (area under curve [AUC] = 0.79) in ROC analysis. The combination of miR‐98‐5p + miR‐139‐5p was seen to be a good indicator (AUC = 0.839). Conclusions These results suggest that alteration of the miRNA levels can be used as auxiliary parameters to explain the pathophysiology of OAB syndrome and could shed light on new treatment options.

Purpose: The aim of this study was to evaluate changes in levels of adrenergic receptor β3 (ADRB3), Rho-related kinase 2 (ROCK2), and guanine nucleotide exchange factor (GEF), which play key roles in the adrenergic and cholinergic pathways of contraction-relaxation harmony in voiding physiology, and to explore the relationship between these proteins and overactive bladder (OAB).Methods: This study included 60 idiopathic OAB patients and a healthy control group. A validated OAB-validated 8 questionnaire was completed by all participants. Serum levels of ADRB3, ROCK2, and GEF were examined with enzyme-linked immunosorbent assays. Patient and control groups were compared in terms of these levels, and receiver operating characteristic (ROC) curves were generated for all parameters.Results: The levels of ROCK2 were significantly elevated, but there were no correlations between the OAB symptom score and the serum levels of ROCK2, ADRB3, and GEF in OAB patients. In the ROC analysis, ROCK2 alone provided the strongest potential relationship (area under the curve=0.651) with 84.9% sensitivity. The ROCK2+GEF combination provided a satisfactory relationship (AUC=0.755). The AUC for the ADRB3+ROCK2+GEF combination was 0.752, with 64.2% sensitivity and 88.2% specificity.Conclusions: The study results suggest that alterations in serum ROCK2 levels and the use of this parameter in combination with ADRB3 and GEF levels can shed light on the pathophysiology of idiopathic OAB syndrome and provide a new perspective for treatment.

Aim: The aim is to evaluate the compatibility of sodium, potassium, and glucose measurement procedures of point-of-care (POC) blood analyzer and biochemistry auto-analyzer.Methods: Sodium, potassium, and glucose test results performed in our laboratory between 01-30 March 2021 were obtained retrospectively. Sodium, potassium and glucose tests were analyzed simultaneously (within half an hour) in the auto-analyzer, and the POC blood analyzer was included in this study. The compatibility between the POC blood analyzer and auto-analyzer results was evaluated by Passing-Bablok regression analysis and Bland-Altman plots.Results: Passing-Bablok regression were y=-33.25+1.25x, y=0.35+1x, and y=-4,182+1.045x for sodium, potassium and glucose, respectively. There was no significant deviation from linearity for sodium, potassium, and glucose (p=0.50, p=0.68, and p=0.48 respectively). The mean absolute difference were -1 mmol/L (95% CI=-7 to 5), -0.42 mmol/L (95% CI=-1.38 to 0.54), and -3.5 mg/dL (95% CI=-35.4 to 28.3) for sodium, potassium and glucose, respectively.Conclusion: Sodium, potassium, and glucose measurement method procedures measured in the POC blood analyzer and auto-analyzers are compatible. Random distribution of differences between values measured by both methods around zero (not showing a systematic distribution) showed a good fit between the methods. The calculated bias values do not exceed limits determined by Clinical Laboratory Improvement Amendment (CLIA). According to the data we have obtained, reporting that the results obtained with the POC blood analyzer are compatible with the test results of the automated analyzer and sharing these results with clinicians will contribute to the effective use of the test results obtained with the POC blood analyzer for patients.

ObjectivesCirculation has an essential role in tissue healing. The increased blood circulation facilitates the delivery of protein, nutrients, and oxygen, which in turn results in better tissue healing. Previous studies have compared the effects of different physiotherapy treatment methods including physical agents, massage and exercises on the peripheral circulation to determine their effectiveness. Harmonic technique is recent name given to a group of manual techniques involving gentle rocking of different body masses. The name ‘harmonic technique’ is derived from that of a physical phenomenon called harmonic motion. This describes the rhythmic and cyclical motion of an object motion between two spatial positions. The aims of the study were to investigate the effect of active assistive and passive harmonic therapy movements on acute peripheral blood circulation in young males and to compare with passive range of motion.MethodsEighteen young males with 22.17 ± 1.68 years average age (age range:20–25) participated in the study. 83.3% of males were right-handed. Active assistive harmonic therapy (AHT), passive harmonic therapy (PHT) and passive range of motion (PROM) were applied on shoulder joint of dominant upper extremity in three consecutive sessions one week apart. Duration of a session was 10 minutes. All interventions were applied in the sitting position. The same physiotherapist applied all interventions. Pre-and post-movement period, Doppler ultrasonography used for peripheral blood circulation measurement from radial artery in both dominant and non-dominant side and peripheral blood circulation was calculated by using an equation. Doppler ultrasonography measurements were performed by the same radiologist and radiologist was blind about intervention.ResultsAfter AHT and PHT applications, blood flow of radial artery increased (p < 0.05), whereas there were no statistically significant differences in PROM group at dominant side (p > 0.05). No statistically significant differences were found between blood flow of radial artery at pre- and post- intervention sessions for non-dominant sides in the all groups (p > 0.05).Abstract P-5 Table 1Comparison of pre and post-intervention blood flow of radial artery in the groups TAMAX AHT PHT PROM Pre-Post X±Ss (p) Pre-Post X±Ss (p) Pre-Post X±Ss (p) Dominant extremity 20.84 ± 6.81–22.57±6.14 (0.044) 10.79 ± 3.76–26.35±7.47 (0.000) 14.45 ± 5.93–15.80±6.89 (0.255) Non-dominant extremity 21.42 ± 8.64–22.16±8.82 (0.495) 22.11 ± 7.22–21.90±6.94 (0.806) 15.90 ± 7.21–14.06±6.10 (0.072) TAMAX: Time-averaged maximum velocityAbstract P-5 Table 2Comparison of between groups in terms of percentage values for time-averaged maximum velocity (TAMAX). TAMAX AHT PHT PROM X±Ss X±Ss X±Ss f p Dominant extremity 1.72 ± 3.256.25 ± 5.241.35 ± 4.866.400.003a,b Non-dominant extremity 0.73 ± 4.34-0.21 ± 3.59-1.83 ± 4.071.850.167aAHT vs PROM, bPHT vs PROM.ConclusionsBoth active assistive and passive harmonic therapy increased peripheral blood flow in young mal...