Fungal biotransformation is a pertinent strategy to overcome difficulties and problems arising from chemical synthesis and direct extraction from nature. This biotechnological approach is a relevant strategy to obtain high-added-value aroma compounds under environmentally friendly conditions. In order to understand the effect of an amazon endophytic fungus on the monoterpene substrate, this research work aims to investigate the biotransformation using cells cultivated of Pestalotiopsis versicolor LabMicrA-478 with R-(+)-limonene as a sole carbon and energy source. The main products of the limonene biotransformation identified by gas chromatography-mass spectrometry (Thermo Scientific™) with the NIST database were limonene-1,2-diol (74,97%) and limonene-1,2-epoxide (1.94%) in 120 hours of reaction. Finally, this is the first report to characterize the bioconversion of R-(+)-limonene by P. versicolor LabMicrA-478, as a biocatalyst.