Mice injected with a single dose of 60 mg cholesterol oleate emulsion showed substantial blockade of the mononuclear phagocyte system, measured by the rate of vascular clearance of radio-labelled sheep erythrocytes. The Unanue, 1972;. Physiological investigation under in vivo conditions is made difficult by the scattered distribution of lymphoid tissues and the intermingling of macrophages with lymphocytes in these tissues, and the susceptibility of both cell types or their precursors to irradiation and cytotoxic agents. Nevertheless, as stressed by Gorczynski, Miller and Philips [1971], there is a need to test the inferences derived from in vitro studies by in vivo experiments.Modification of the phagocytic function of fixed mononuclear phagocytes is readily achieved by the intravenous injection of certain simple lipids [Di Luzio and Blickens, 1966;Stuart, 1970]. Emulsions of cholesterol oleate injected intravenously block the mononuclear phagocyte system for several days and have not been observed to have any effect on the ultrastructure of lymphocytes of the spleen and lymph nodes . No effect on cultured lymphocytes can, as yet, be attributed to treatment with this lipid .