AbstrakPenelitian ini bertujuan untuk menganalisis dosis optimal induksi IL-6 pada tikus hamil yang dapat memicu peningkatan tekanan arteri dan protein sebagai dua gejala preeklampsia. Sebanyak 25 tikus hamil dibagi menjadi 5 kelompok, yaitu tikus hamil kelompok kontrol (tanpa induksi IL-6), 1 kelompok tikus hamil diberi induksi IL-6 dalam dosis 1,25 ng / hari, 1 kelompok tikus hamil diberi induksi IL-6 dosis 2,5 ng/ hari, 1 kelompok tikus hamil diberi induksi IL-6 dosis 5 ng / hari, dan kelompok tikus hamil diberi induksi IL-6 dosis 10 ng / hari. Induksi IL-6 dilakukan pada hari kesepuluh kehamilan selama 5 hari. Ekspresi caspase-3, IL-17, dan STAT3 dianalisis dengan mikroskop confocal. Ekspresi caspase-3, IL-17 dan STAT3 secara signifikan lebih tinggi pada kelompok preeclampsia dibandingkan kelompok kontrol (p <0,05). Ekspresi caspase-3 menurun secara signifikan pada semua kelompok yang diobati dengan phycocyanin dibandingkan kelompok preeklampsia (p <0,05), namun belum dapat mencapai ekspresi yang sebanding dengan kelompok kontrol (p <0,05). Ekspresi IL-17 menurun secara signifikan pada kelompok tersebut dengan pemberian dua dosis tertinggi phycocyanin dibandingkan dengan kelompok preeklamsia (p <0,05). Ekspresi STAT3 menurun secara signifikan pada semua kelompok yang diberi phycocyanin dibandingkan kelompok preeklampsia (p <0,05), mencapai ekspresi yang sebanding dengan kelompok kontrol pada kelompok yang menerima phycocyanin pada dosis 10 dan 20 ng (p> 0,05). Kesimpulannya, IL-6 pada tikus hamil mampu meningkatkan apoptosis melalui jalur IL-17 dan STAT. Penghambatan apoptosis akibat pengobatan phycoyanin tidak hanya melibatkan pembentukan IL-17, data ini ditemukan dalam dosis phycocyanin 10 dan 20 ng. AbstractThis study was to analyze the optimum dose of IL-6 induction in pregnant rats that could trigger an increase in mean arterial pressure and protein as two symptoms of preeclampsia. A total of 25 pregnant rats was divided into 5 groups, including pregnant rats the control group (without induction of IL-6), a group of pregnant rats were given an induction in IL-6 doses of 1.25 ng/day, a group of pregnant rats given doses of IL-6 induction 2.5 ng/day, a group of pregnant rats were given an induction of IL-6 doses of 5 ng/day, and groups of pregnant rats were given an induction in IL-6 doses of 10 ng/day. Induction of IL-6 was performed on the tenth day of gestation for 5 days. The expression of caspase-3, IL-17, and STAT3 were analyzed by confocal laser scanning microscopy. The expression of caspase-3, IL-17 and STAT3 was significantly higher in preeclampsia group than the control group (p<0.05). The expression of caspase-3 decreased significantly in all groups were treated by phycocyanin compared to the preeclamptic group (p<0.05), but has not been able to reach expression comparable to the control group (p<0.05). Expression of IL-17 decreased significantly in the group given the two highest doses of phycocyanin compared to the preeclampsia group (p<0.05). STAT3 expression decreased significantly in ISSN 1...

Preeclampsia is a pregnancy disorder with hypertension as one of its common symptoms. Preeclampsia in pregnancy is characterized by increasing of proteinuria, blood pressure and also interleukin 6. Spirulina sp is a thread like shaped blue-green algae, similar to chain of cylindrical cells with 1 to 12 μm diameter of cell membrane. The purpose of this research was to know the effect of spirulina on VEGF level in improving of trophoblast function in pregnant white Rats Wistar. The design of the study was laboratory experimental with post-test only control group design. Twenty-five rats with preeclampsia model induced by interleukin 6, were divided into 5 groups. The groups were control group, positive control group (P0) injected by interleukin-6 dose of 5 ng/ 100 gram body weight (BW), group with spirulina dose of 10 mg/100 gram BW (P1), group with spirulina dose of 20 mg/100 gram BW (P2); and group with spirulina dosage 40 mg/100 gram BW (P3). The Vascular endothelial growth factor (VEGF) levels were in experimental groups measured compared to control. The averages of group spirulina were 81.31 (10 mg/100 gram BW), 65.99 (20 mg/100 gram BW), and 49.62 (40 mg/100 gram BW). In comparison with control group (62.70), VEGF level in group administered by spirulina dose of 20 mg/100 gram BW were close to control group.

Introduction: Diseases that accompany pregnancy, such as diabetes mellitus, affect preeclampsia. This disease is a hereditary disorder characterized by reduced circulating insulin, high blood sugar concentrations, and reduced glycogenesis. This study tried to determine the effect of giving Moringa leaf powder (Moringa oleifera) in preventing pancreatic organ cell damage in pregnant rats with diabetes mellitus. Method: A total of 30 pregnant white rats, which were divided into 6 groups, were examined for their sugar levels on day 4 after being induced by alloxan for 18 days to ensure that they were already in a hyperglycemic state. Result: The results showed that the administration of Moringa leaf powder at a dose of 800 mg/day/kg BW could reduce pancreatic cell apoptosis, approaching the negative control group. Conclusion: In conclusion, the moringa leaf powder is able to improve the clinical pathological condition of pregnancy, due to inhibition of apoptosis and repair of pancreatic Langerhans cells.

Preeclampsia is multisystem specific disorder in pregnancy. Preeclampsia characterized by increased cytokine Interleukin-6 and β hCG (human Chorionic Gonadotropin). Spirulina is green-blue alga contain antioxidant, vitamin, mineral. Spirulina has a potential effect as antiinflammation. The aim of this research was to know effect of spirulina to repair trophoblast at β hCG level of pregnant rats with preeclampsia condition (biology material collecting). The type of this research is an experimental laboratory with post-test only control group design. Preeclampsia models induced by Interleukin-6. Twenty-five rats were grouped to five group: Control, Control Positive, Spirulina dose 10, 20, and 40 mg/day, at the end of treatment, β hCG level were analyzed. Statistical analysis was used by one-way ANOVA. The results of this study showed that β hCG level of group spirulina dose 10 mg/day (85.11 ± 25.70 mIU/ml) did not significantly different (p=0.353), with group Dose 20 mg/day (79.65 ± 10.65 mIU/ml). Level of β hCG in Spirulina group dose 40 mg/day were 93.28 ± 17.12 mIU/ml. The spirulina groups dose 10 mg/day and dose 40 mg/day did not show significantly different level of β hCG (0.730> 0.05). Administration of spirulina at a dose of 10 mg was able to significantly reduce levels of β hCG (P <0.05) than at doses of 20 mg / day and 40 mg/ day.

The placenta is a joint organ of the uterus surface mother and fetus. One of cytokines of the placenta is IL-6 which is essential for normal placental development and successful pregnancy. Sgp130 binds Interleukin-6-sIL-6R complex, so Interleukin-6 signal cannot be passed on. Both factors play an important role in pre-eclampsia which has high levels of IL-6 and sgp130. They are expected to be suppressed by administration of C-phycocyanin. The aim of this study to prove effect of C-phycocyanin on sgp130 levels among wistar rats’ trophoblasts exposed to IL-6. The design of the study was Experiment, Post Test Only Control, analyse with One way Anova. The samples were 25 rats divided four treatment groups. Dose of C-Phycocyanin (10mg, 20mg, 40mg). The results significant difference between control and treatment group IL-6, IL-6 + CPC10, IL-6 + CPC20. But there was no significant difference between control group and IL-6 + CPC40, sig value. 0.214 (sig. <0.05). Conclusion. There is an effect of C-Phycocyanin on Sgp130 levels in wistar rats’ trophoblasts exposed to IL-6 at a dose of C-Phycocyanin 40mg.