Type 2 diabetes is traditionally diagnosed by the use of an oral glucose tolerance test and/or HbA1c, both of which require serum collection. Various biomarkers, which are measurable biological substances that provide clinical insight on disease state, have also been effective in the early identification and risk prediction of inflammatory diseases. Measuring biomarker concentrations has traditionally been obtained through serum collection as well. However, numerous biomarkers are detectable in saliva. Salivary analysis has more recently been introduced into research as a potential non-invasive, cost-effective diagnostic for the early identification of type 2 diabetes risk in adults and youth. Therefore, the purpose of this review was to compare 6 established inflammatory biomarkers of type 2 diabetes, in serum and saliva, and determine if similar diagnostic effectiveness is seen in saliva. A lack of standardized salivary analysis, processing, and collection accounts for errors and inconsistencies in conclusive data amongst studies. Proposing a national standardization in salivary analysis, coupled with increased data and research on the utility of saliva as a diagnostic, poses the potential for salivary analysis to be used in diagnostic settings.
Type 2 diabetes (T2D) is a chronic metabolic disorder characterized by a progressive loss of ß-cell function relative to insulin resistance. Once thought to be an adult disease, T2D has emerged as an increasingly prevalent health concern in obese youth, suggesting that early identification of those who are at high-risk for T2D is critical. Traditionally, either fasting glucose and/or 2-hr glucose concentrations during an oral glucose tolerance test has been used to identify prediabetes (i.e., intermediate stage of T2D). However, the fact that multiple blood collections are required during the test hinders necessary screening of high-risk youth with obesity and metabolic syndrome, primarily due to the invasive methods (i.e., fear of needles). For this reason, there is increasing interest in salivary biomarkers that can identify metabolic abnormalities. Goodson et al. provided critical evidence for future use of salivary samples for T2D risk detection as investigating saliva-based inflammatory biomarkers in an at-risk population of children. A total of 744 children (mean age 11 years) were divided into 4 groups based on their weight status using the age- and sex-standardized BMI z-score: underweight, normal healthy weight, overweight, and obese (n=186 for each group). Fasting saliva samples were collected from all participants to examine 20 biomarkers and the biomarkers were analyzed on a Luminex 200 platform (Luminex Corporation, Austin, TX, USA). Of the 20 biomarkers tested, 16 showed no significant differences between obese and normal weight subjects. However, 4 biomarkers did show significant differences between obese and normal weight subjects. Salivary C-reactive protein (CRP), a pro-inflammatory biomarker, was 6 times higher in obese youth compared with their normal weight peers (median 435 pg/ml vs. 76 pg/ml, P<0.0001) (Table 1). Salivary insulin and leptin showed a 3-fold increase in obese children compared with their normal weight counterparts. Salivary adiponectin (anti-inflammatory biomarker and indicator of insulin sensitivity) concentration was 30% lower in overweight (median 2,981 pg/mL) and obese (2,798 pg/mL) youth compared with normal weight (4,083 pg/ml, P<0.0001) (Table 1). Biomarkers provide essential information for understanding the etiology of diseases, treatment, and early intervention. Saliva sampling has been introduced into research as a potential indicator of T2D risk and correlations between serum and saliva samples have been identified. In this study, 4 biomarkers were identified that exhibit significant change with increasing body weight in a pediatric population (Goodson et al., 2014). This suggests that saliva could be a useful blood replacement for the study of metabolic complications of obesity in children. In the face of COVID-19, this study is especially important in bringing awareness to the possible use of salivary biomarkers in determining potential risk for T2D and metabolic syndrome. With the limited in-person human research available currently, a new non-invasive, cost-effective, time-saving method is of the utmost importance in addition to the fact that at-home saliva collection is feasible for the research purpose (https://salimetrics.com/how-do-i-collect-saliva/). Although Goodson et al. proved the utility of the saliva analysis for T2D risk, a lack of validation data against blood samples is considered significant obstacle for a solid conclusion from this study. The saliva samples need to be compared to their respective blood samples in order to validate its reliability as novel biomarker. Furthermore, it would be germane to see if salivary biomarkers can be used for testing effectiveness of any prevention and treatment of metabolic syndrome and T2D in youth populations.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Copyright © 2025 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.