Objective: To evaluate the impact of tooth loss on the quality of life of patients at the Cesmac University Center. Material and Methods: A cross-sectional study was performed, whose sample was composed of 224 volunteers from various health units of the selected health service with at least 12 years of age and one missing tooth. Quality of Life (QOL) was assessed using the Oral Health Impact Profile (OHIP -14) and tooth loss was assessed with the dental chart. A structured interview that assessed the socioeconomic condition of the individual was also applied. For interpretation of the OHIP-14, values were assigned using the multiplicative method: 0-3 points = no impact; 3.01 to 6 points = low impact; 6.01 to 10 points = moderate impact; and> 10.01 points = high impact. All volunteers received guidance on oral health and, when necessary, a referral to the dental care provided by this institution was performed. Results: The mean OHIP ranged from no impact (30.9%) to high impact (27.8%). The correlation between the number of missing teeth and QOL scores was statistically significant (p <0.05), as well as the correlation between number of missing teeth and age of volunteers (p <0.0001). The dimensions of the OHIP-14 that showed the most influenced domains were pain, psychological discomfort, psychological disability. Conclusion: Increasing age has shown influence on tooth and tooth loss affected the QOL of volunteers. QOL may be influenced by other factors such as loss of anterior teeth and schooling.