Banana is a fruit plant that is widely produced in Indonesia. Unfortunately, this plant is very susceptible to diseases which can reduce the quality and quantity of the crop. This paper proposes disease detection in banana plants using a thermal camera. The detection is carried out using image processing techniques with multilevel thresholding methods. The image is captured using a thermal camera, then the image is preprocessed to suit what is desired. After that, so that the position is the same as the image taken using a digital camera, the image produced by the thermal camera is carried out by an image registration process. The image processing result is compared with the ground truth image obtained from a digital camera to determine the effectiveness of the proposed method. The effectiveness of the proposed method is measured using the parameters Recall, Precision, F-measure, and Accuracy. The effectiveness of the proposed method is quite effective because it produces parameter values above 80%, namely the recall value of 86,59%, the Precision of 99,1%, the F-measure of 92%, and the accuracy of 89,78%.