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Phosphor thermometry is a promising approach for remote and non-intrusive two-dimensional temperature sensing. However, the characteristics of each phosphor material have to be carefully evaluated to avoid measurement inaccuracies. We characterized the luminescent properties of the thermographic phosphor particles YAG:Dy,Er seeded to the gas flow in a flow cell. The particles were excited by a laser sheet of a 355 nm Nd:YAG laser, and two intensified cameras were used for two-color detection of the phosphorescence spectrum. The calibration cell enables homogeneous and repeatable measurement conditions. A newly designed seeder allowed to adjust the number of particles in the cell, achieving sufficient seeding densities even at low flow rates in a wide temperature range. Additionally, the influence of the seeding density and the laser fluence on the intensity ratio were studied, both having negligible effects on the estimated temperature.