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A lysine-deficient basal diet, both with and without supplemental lysine added, was fed to groups of Barred Plymouth Iiock cockerels from hatching to 4 weeks of age. .4t this time blood sanlples were taken from the carotid artery and pooled for each group. Twelve amino acids were determined in the deproteinized plasma by microbiological assay. Deproteinized plasma from birds receiving the lysine-deficient diet was lower in lysine content and higher in threonine and tyrosine than that from birds fed the lysine-supplemented diet. Other amino acids showed milch smaller differences. A similar amino acid pattern was observed when the birds were fed the lysine-supplemented diet for 25 days from hatching and then the lysine-deficient diet for 3 days before blood samples were taken. Withholding feed for 24 hours before the samples were taken resulted in a marked increase in Iysine and threonine concentration, an effect which has been reported by other workers. Acid hydrolysis of the deproteinized plasma increased somewhat the concentration of lysine, as measured microbiologically, bt!t had no effect on, or solnewhat reduced, the concentrations of other a~nino ac~ds.

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