The number of occurrences of maned wolf in the Atlantic Forest has increased in the last decade. However, records of Chrysocyon brachyurus for lowland Atlantic Forest localities are rare. Here, we present an unprecedented record of a maned wolf in the northern coast of the state of São Paulo, Brazil, victim of vehicle collision on the Rio-Santos road. This record is related to habitat loss due to changes in the use of the Cerrado's soil and to the increase in open areas in the Atlantic Forest. These changes facilitate the dispersion of the maned wolf and bring it closer to humans, increasing the chances of accidents such as collisions with motor vehicles.