The whitefly-transmitted Tomato Yellow Leaf Curl Virus (TYLCV) is the major pathogen of tomato crop in Cuba and one of the most outstanding viral diseases of plants worldwide. In this work, we have developed transgenic tomato plants, transformed with an intron-hairpin genetic construction to induce post- transcriptional gene silencing against the early TYLCV replication associated protein gene (C1). The intron-hairpin RNA produced involves 726 nts of the 3' end of the TYLCV C1 gene as the arms of the hairpin, and the castor bean catalase intron. Transgenic tomato plants belonging to line 126, which harbor a single transgene copy, showed immunity to TYLCV, even in extreme conditions of infection (4-leaf-stage plants and 300 to many hundreds viruliferous whiteflies per plant during 60 days). Dot blot hybridization of these plants showed no TYLCV DNA presence 60 days after inoculation. Small interfering RNA molecules were detected in both inoculated and non-inoculated plants from line 126. These transgenic tomato plants of the otherwise very TYLCV-susceptible Campbell-28 tomato cultivar, are the first report of resistance to a plant DNA virus obtained by the use of the intron-hairpin RNA approach.
Предлагается заменить коды Гоппы кодами $\mathrm{QC}$-$\mathrm{LDPC}$ в схеме электронной подписи $\mathrm{mCFS}$. Это дает значительное (в среднем в $50$ раз) сокращение размеров открытых ключей без потери стойкости. Используется такая же теоретическая модель, как для схемы $\mathrm{mCFS}$. Обсуждаются возможности использования ряда известных методов для атаки на хэш-функцию и на процедуру выработки открытого/закрытого ключей. Предложены наборы параметров для нескольких уровней стойкости, например $80$-битовый уровень стойкости достижим с открытым ключом размера $\approx 1.82$ KB, $128$-битовый уровень стойкости - с ключом $\approx 3.87$ KB и $256$-битовый уровень стойкости - с ключом $\approx 13.88$ KB.
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