Introduction: Penile length is very important for evaluation of the normal external genitalia and detection of genital anomalies such as macro-and micro-penis. Establishing a normal population, age specific reference range for penile length helps in both accurate evaluation of penile size and early detection of any penile abnormalities Objective: To assess normal values of stretched penile length of the prepubertal normal Egyptian boys aged 5 to 9 years and to establish a penile growth chart & percentiles concerning penile length for those boys. Patients and Methods:A cross sectional study is conducted in Egypt included 1500 boys aged 5 to 9 years in Cairo University Children's Hospital, Faculty of Medicine. Boys are divided into 5 groups each group 300 boys. All boys were subjected to measurement of stretched penile length, height, weight, and BMI. Results: The mean penile length ± SD by age grouping were at 5 years = 5.31 ± 0.92 cm, at 6 years = 5.51 ±1 cm, at 7 years = 5.87 ± 0.85 S cm, at 8 years = 6.19 ± 0.93 cm and at 9 years = 6.1 ± 0.92 cm. Conclusion:In order to aid in the diagnosis of genital abnormalities, this study introduced new reference values, percentiles and chart for stretched penile length in prepubertal normal Egyptian boys aged 5 to 9 years.