Quinoa (Chenopodium quinoa Willd.) is a halophytic crop that can withstand a variety of abiotic stresses, including salt. The present research examined the mechanisms of salt tolerance in five different quinoa genotypes at four different salinity levels (control (60), 80, 120, and 160 mM NaCl). ISSR and SCoT analysis revealed high polymorphism percentages of 90.91% and 85.26%, respectively. Furthermore, ISSR 1 and SCoT 7 attained the greatest number of polymorphic amplicons (27 and 26), respectively. Notably, LINE-6 and M-28 genotypes demonstrated the greatest number of unique positive and negative amplicons (50 and 42) generated from ISSR and SCoT, respectively. Protein pattern analysis detected 11 bands with a polymorphism percentage 27.27% among the quinoa genotypes, with three unique bands distinguishable for the M-28 genotype. Similarity correlation indicated that the highest similarity was between S-10 and Regeolone-3 (0.657), while the lowest similarity was between M-28 and LINE-6 (0.44). Significant variations existed among the studied salinity treatments, genotypes, and the interactions between them. The highest and lowest values for all the studied morpho-physiological and biochemical traits were recorded at 60 and 160 mM NaCl concentrations, respectively, except for the Na and proline contents, which exhibited the opposite relationship. The M-28 genotype demonstrated the highest values for all studied characteristics, while the LINE-6 genotype represented the lowest in both seasons. On the other hand, mRNA transcript levels for CqSOS1 did not exhibit differential expression in roots and leaf tissues, while the expression of CqNHX1 was upregulated more in both tissues for the M-28 genotype than for the LINE-6 genotype, and its maximum induction was seen in the leaves. Overall, the genotypes M-28 and LINE-6 were identified as the most and least salinity-tolerant, respectively.
Six field experiments were carried out in 2015 -16 and 2016 -17 seasons. A randomize complete block design with three replications was used at three locations :1. Giza Experiment Station (latitude 30.01 0 N and longitude 31.21 0 E); 2. Tamiya District, Fayoum Governorate (latitude 29.30 0 N and longitude 30.84 0 E) and 3. Ismailia Governorate (latitude 30.36 0 N and longitude 32.16 0 E)]. The performance of six sugar beet varieties (cvs.); Samba, Pleno, Gloria, Poly belga, Oscar poly and Gazella was studied to estimate sugar yield and its contributing traits as well juice quality traits under the three environmental conditions. The obtained results showed that growing seasons had a significant effect on impurities (K, Na and α-amino N), some technological characteristics (purity, extractable sugar, sucrose and sugar lost to molasses percentages) as well sugar yields/fed. The effect of varieties and locations factors significantly affected sugar percentage, extractable and sugar yield. The obtained results indicated that sowing Samba variety in the three locations had the highest sugar extraction and sugar percentages as well as sugar yield/fed. In general, results revealed that Samba variety followed by Gazella is favorable for the regions of Giza, Fayoum and Ismailia Governorates.
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