Antibiotics determination plays a major role in minimizing antimicrobial resistance starting from quality control of pharmaceutical formulations to therapeutic drug monitoring. Green modified glassy carbon electrode has been developed for determination of tedizolid phosphate; new antibiotic prodrug; in presence of its active metabolite. The graphene transducer interlayer, dispersed with PVC, improved the electrode stability and standard potential reproducibility. Graphene hydrophobicity prevented the water layer formation between the sensing layers that decreased the potential drift down to 267 μV h−1. Electrochemical impedance showed a low resistance value for graphene containing sensor due to its high electron transfer ability.
Tedizolid phosphate is an antibiotic prodrug that is metabolized into tedizolid which is used against various resistant bacterial strains. In this study, tedizolid phosphate was subjected to stress degradation conditions, namely, hydrolysis (neutral, acidic and alkaline), thermal, oxidative and photolytic ones. The prodrug was stable toward thermal and photolytic stress conditions, while it showed significant degradation upon applying oxidative and hydrolytic conditions. Two suggested chromatographic methods are described for separation and determination of tedizolid phosphate from the resulted degradation products. The first method is HPLC using Waters Xselect HSS C18 (250 × 4.6 mm, 5 μm) analytical column and mobile phase composed of phosphate buffer (50 mM, pH 6.5):acetonitrile (70:30, %v/v) pumped at flow rate of 1.0 mL/min with UV-detection at 300 nm. The second method is a TLC coupled with densitometric quantitation, precoated silica TLC-plates as a stationary phase and a mobile phase of methanol:butanol:ethyl acetate:ammonia (33%, w/v) (60:20:20:10,%v/v) were used. The chromatographed plates were scanned at 300 nm. The linearity was confirmed over concentration range of 1–100 μg/mL and 1–12 μg/band for HPLC and TLC-densitometric methods, respectively. Both methods were found to be suitable for determination of tedizolid phosphate in pure form and in its pharmaceutical formulations.
Solid contact sensors were designed for determination of the antibiotic prodrug tedizolid phosphate. The sensors are based on; microfabricated copper as costeffective electrode substrate and graphene layer as a transducer. The ion sensing PVC polymeric membrane was optimized by proper selection of the anion exchanger. The graphene layer was characterized by scanning elec-tron microscopy, Raman spectroscopy, FT-IR, besides measuring electrochemical impedance for the fabricated sensors. The potential drift decreased down to 0.133 mV h À 1 compared to 6.67 mV h À 1 for graphene free sensor. The sensors have been used to determine tedizolid phosphate in its pharmaceutical formulation and content uniformity testing applications.
Background Green chemistry approach was developed for the purpose of saving the environment by using green solvents. Applying the green analytical chemistry principles for traditional methods is considered a challenge. Acemetacin is a commonly used analgesic prodrug that bioactivated to indomethacin. Objectives Developing two simple Eco-friendly chromatographic methods for simultaneous determination of acemetacin and indomethacin. Methods The first is HPLC-DAD method, separation was performed on Waters XBridge® Shield RP18 (250 × 4.6 mm, 5 µm) analytical column with ethanol–ammonium acetate buffer (50 mM, pH 3.5 ± 0.1) (60:40, v/v) as a mobile phase at a flow rate of 1 mL/min at 25 ± 0.5 °C and UV detection at 254 nm. The other method is TLC method coupled with densitometric quantification using pre-coated silica TLC-plates and butanol–ethyl acetate (70:30, v/v) elution system. The plates were scanned at 254 nm. Results Both methods were validated according to the ICH guidelines, linearity was confirmed, for both, over a concentration range of 1–100 µg/mL for the HPLC method and 0.2–7 µg/band for TLC-densitometric one. Methods’ greenness was evaluated by the National Environmental Methods Index, Eco-Scale, Green Analytical Procedure Index metrics and Analytical GREEnness Metric Approach. Conclusion The proposed methods were found to be suitable for determination of studied drugs in their marketed formulations and suggested for routine analysis in quality control laboratories. Highlights HPLC and TLC methods were validated for the assay of acemetacin and indomethacin. Methods’ greenness was evaluated by NEMI, Eco-Scale, GAPI and AGREE metrics and were compared to published methods. The developed HPLC method shortened the elution time of the analyzed drugs saving more time and money and the TLC method lowered the drugs’ detection limit.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.